Table 2.

Effect of wild-type and mutant Hrs over-expression on EGFR retention

Distribution of EGFR over the endosomal vacuole (%) Number of internal vesicles per vacuole
Limiting membrane Internal vesicles Total number Number of vacuoles
Control 16±5.6 84±5.6 161 74 7±1.3
Hrs 77±8.2 23±8.2 401 159 3±1.1
Hrs-Y329/334F 90±3.5 10±3.5 291 141 3±0.3
Hrs-ΔUIM 57±2.2 43±2.2 294 107 10±3.0
  • The relative distribution of EGFR over endosomal vacuoles was determined by counting gold particles (total number indicated) for each transfection of at least 3×10 cell profiles (see Materials and Methods) and expressed as percentage of the total label present on vacuoles. For each EGFR-positive vacuole, the number of internal vesicles was counted. All values are mean±standard deviation.