Table 1.

Quantification of mitotic phenotypes at different times after depletion of DmSMC4 by RNAi

Chromatin bridges
*Unresolved sister chromatids (%) Anaphase (%) Telophase (%)
Time (h) Control RNAi Control RNAi Control RNAi
24 0 28.6 0 0 0 6.3
48 0 54.5 0 25.0 0 4.0
72 0 73.7 0 46.2 0 50.1
96 0 97.2 0 61.5 0 73.7
120 0 100 0 50.0 0 89.5
144 0 100 0 85.7 0 94.4
  • * Cells in prometaphase. Total number of cells counted for controls: 24 hours n=1768, 48 hours n=2041, 72 hours n=2304, 96 hours n=2724, 120 hours n=2066 and 144 hours n=1146. Total number of cells counted for RNAi-treated cells: 24 hours n=1792, 48 hours n=1958, 72 hours n=2270, 96 hours n=2888, 120 hours n=1902 and 144 hours n=2091