Table 2.

Labeling of different cell regions by pEg 7G antibodies

Mitotic chromosomesCytoplasmKaryoplasmNucleolus
Met 6 minutes, -20°C+++f gr+f gr-++
Met 6 minutes, -20°C, Trit 3 minutes RT+++f gr+dif -+++
Met 6 minutes, Ac (6 or 10 or 20 minutes), -20°C+++f gr+dif -++
Met 6 minutes, Ac 20 minutes -20°C, Tr 3 minutes RT+++f gr+dif -+++
Met/Ac (6, 10 or 20 minutes), -20°C++++l gr +dif -++
Met/Ac (6, 10 or 20 minutes), -20°C, Tr 3 minutes RT+++l gr -+f gr +++
Tr 3 minutes, RT Met 6 minutes -20°C++++f gr -+f gr ++++
Tr 3 minutes RT, Met 6 minutes, Ac 20 minutes, -20°C++++f gr -+f gr ++++
Tr 3 minutes, For 3% 30 minutes RT+++++f gr -+f gr ++++
Tr 3 minutes, For 3%/Glu 0.1%, NaBH4 2×10 minutes, RT++++-dif —+-
Tr 3 minutes, Glu 1%, NaBH4 2×10 minutes, RT++++-dif —+-
For 3% 10 minutes, Tr 2×5 minutes++++-f gr +-
  • Abbreviations: Met, methanol 100%; Tr, Triton X-100 1%; For, formaldehyde; Glu, glutaraldehyde; NaBH4, 2 mg/ml in PBS; f gr, fine granular labeling; l gr, large granular labeling; dif, diffuse labeling.