Table 3.

Labeling of different cell regions by XCAP E1 antibodies

Mitotic chromosomesCytoplasmKaryoplasmNucleolus
Met 6 minutes, -20°C++f gr ++-+
Tr 3 minutes, RT Met 6 minutes -20°C+++f gr ++-+++
r 3 minutes RT, Met 6 minutes, Ac 20 minutes, -20°C+++f gr -+dif +++++
Tr 3 minutes, For 3% 30 minutes RT+++f gr ++-++++
Tr 3 minutes, Glu 1%, NaBH4 2×10 minutes, RT+-dif -+-
For 3% 10 minutes, Tr 2×5 minutes+++-f gr +++++
  • Abbreviations: Met, methanol 100%; Tr, Triton X-100 1%; For, formaldehyde; Glu, glutaraldehyde; NaBH4, 2 mg/ml in PBS; f gr, fine granular labeling; dif. diffuse labeling.