Table 1.

Localization patterns of AtMinD1-GFP fusion proteins in tobacco leaf cells *

Number of speckles per chloroplast (%) Total number of chloroplasts (%)
Protein Number of cells 0 1 2 3 4 >5
AtMinD1 40 58 (15.8) 223 (60.9) 47 (12.8) 13 (3.6) 9 (2.5) 16 (4.4) 336 (100)
AtMinD1 (A296G) 56 0 (0.0) 140 (31.9) 91 (20.7) 65 (14.8) 42 (9.6) 101 (23.0) 439 (100)
  • * The experiment was performed by particle bombardment using sections from single leaf.

  • Fluorescence speckles were counted by Nikon TE2000 microscope and Hamamatsu ORCA-ER cooled CCD camera and OpenLab software system.