Table 1.

Yeast strains used

Strain Genotype Origin*
CBY340 MATa ura3-52 his3Δ200 lys2-801 leu2-3, 112 trp1Δ901 suc2Δ9
CBY678 MATα ura3Δ0 leu2Δ0 lys2Δ0 his3Δ1 met15Δ0 erg2Δ::kan-MX4
CBY745 MATα ura3Δ0 leu2Δ0 lys2Δ0 erg9Δ::kan-MX4 HIS3::PMET3-ERG9
CBY824 SEY6210 vps4Δ::kan-MX4
CBY858 MATα ura3Δ0 leu2Δ0 lys2Δ0 his3Δ::kan-MX4
CBY886 SEY6210 osh1Δ::kan-MX4 osh2Δ::kan-MX4 osh3Δ::LYS2 osh4Δ::HIS3 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3 [osh4-1, URA3]
CBY892 SEY6210 osh1Δ::kan-MX4 osh2Δ::kan-MX4 osh3Δ::LYS2 osh4Δ::HIS3 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3 [osh4-2, URA3]
CBY924 SEY6210 osh1Δ::kan-MX4 osh2Δ::kan-MX4 osh3Δ::LYS2 osh4Δ::HIS3 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3 [OSH4, TRP1]
CBY926 SEY6210 osh1Δ::kan-MX4 osh2Δ::kan-MX4 osh3Δ::LYS2 osh4Δ::HIS3 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3 [osh4-1, TRP1]
CBY928 SEY6210 osh1Δ::kan-MX4 osh2Δ::kan-MX4 osh3Δ::LYS2 osh4Δ::HIS3 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3 [osh4-2, TRP1]
CBY966 CBY340 osh1Δ::kan-MX4 osh2Δ::kan-MX4 osh3Δ::LYS2 osh4Δ::HIS3 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3 [osh4-1, TRP1] [STE6-HA, URA3]
CBY968 CBY340 [STE6-HA, URA3]
CBY994 MATα ura3Δ0 leu2Δ0 lys2Δ his3Δ1 arv1Δ::kan-MX4
CBY1024 MATa ura3Δ0 leu2Δ0 lys2Δ his3Δ1 las17Δ::kan-MX4 [STE6-HA, URA3]
CBY1026 MATa ura3Δ0 met15Δ0 leu2Δ0 his3Δ::kan-MX4 [STE6-HA, URA3]
DDY904 MATα ura3-52 his3Δ200 lys2-801 leu2-3, 112 D. Drubin (University of California, Berkeley, USA)
DDY1438 DDY904 las17Δ::URA3 D. Drubin (University of California, Berkeley, USA)
JRY4130 MATα ura3-52 his4-619 sec18
JRY6306 CBY340 osh2Δ::URA3 osh4Δ::HIS3 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3 Beh et al., 2001
JRY6312 SEY6210 osh1Δ::URA3 osh2Δ::URA3 osh4Δ::HIS3 osh5Δ::LEU2 osh7Δ::HIS3 Beh et al., 2001
JRY6320 CBY340 osh1Δ::URA3 osh2Δ::URA3 osh3Δ::LYS2 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3 Beh et al., 2001
JRY6326 SEY6210 TRP1::PMET3-OSH2 osh1Δ::kan-MX4 osh2Δ::kan-MX4 osh3Δ::LYS2 osh4Δ::HIS3 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3 Beh et al., 2001
RH286-1C MATa ura3 his4 leu2 bar1 sla2/end4-1(ts) Raths et al., 1993
RSY255 MATα ura3-52 leu2-3, 112 Novick and Schekman, 1979
RSY782 MATα ura3-52 his4-619 sec1-1 Novick and Schekman, 1979
SEY6210 MATα ura3-52 his3Δ200 lys2-801 leu2-3, 112 trp1Δ901 suc2Δ9 Robinson et al., 1988
W303-1A MATa ura3-1 his3-11 leu2-3, 112 trp1-1 can1-100 ade2-1 R. Rothstein (Columbia University, New York, USA)
  • * Unless otherwise stated, all strains were created as part of this study.