Table 1.

Different types of intermediate filaments and their movements

Cytoplasmic intermediate filament type Protein studied Cell type Structural form observed Published results n= Time moving (%) Time pausing (%) Antero movements (%) Retro movements (%) Average antero motility (mm/sec) Range antero (μm/sec) Average retro motility (μm/sec) Range retro (μm/sec)
Type I Keratin 13 AK13-1 Particles (dots) Windoffer and Leube, 1999 Mostly Retro 0.002-0.008 0.002-0.008
Keratin 18 PtK2 Squiggles Yoon et al., 2001 37 18* 82 16 84 0.004±0.03 0.002-0.01 0.004±0.03 0.002-0.01
Type II Keratin 5 NEB-1 Particles Liovic et al., 2003 16 >0.1 >0.1
Keratin 8 PtK2 Filaments Yoon et al., 2001 14 0.001±3.33E-05 0.001±3.33E-05
Type III Vimentin BHK-21 Particles Helfand et al., 2003 53 45 55 66 34 0.39±0.24 0.08-1.65 0.43±0.26 0.08-1.40
Squiggles Yoon et al., 1998 32 97 3 0.06±0.03 0.02-0.19 0.06±0.03 0.02-0.19
Filaments Yoon et al., 1998 23 0.004±0.002 0.004±0.002
Vimentin PtK2 Squiggles Yoon et al., 2001 32 70 30 0.06±0.04 0.006-0.15 0.06±0.04 0.006-0.15
Filaments Yoon et al., 2001 29 0.15±0.11 0.15±0.11
Vimentin 3T3 Squiggles Martys et al., 1999 0.25 0.25
Peripherin PC12 Particles Helfand et al., 2003 77 75 25 65 35 0.33±0.24 0.08-1.45 0.30±0.20 0.08-1.54
Squiggles Helfand et al., 2003 50 70 30 62 38 0.31±0.29 0.08-1.21 0.30±0.28 0.08-1.00
Type IV NF-M Rat sympathetic neurons Short filaments Wang et al., 2000 69 27 73 83 17 0.38 0.02-1.21 0.49 0.15-1.82
NF-M Rat sympathetic neurons Short filaments Wang et al., 2001 69 33 67 69 31 0.56 0.15-1.26 0.62 0.17-1.35
NF-M Rat sympathetic neurons Particles Wang et al., 2001 17 56 44 22 88 0.08 0.06-0.10 0.32 0.13-0.60
NF-M NB2a/d1 Particles Yabe et al., 1999 >80% <20% 0.025±0.005
NF-H Rat sympathetic neurons Short filaments Roy et al., 2000 73 20 80 71 29 0.67 0.14-1.7 0.57 0.11-1.4
NF-L, NF-M, NF-H Purified bovine spinal cordrrrr Short filaments Shah et al., 2000 0.27 ∼0.1-0.85 0.29 ∼0.1-1
NF-L, NF-M, NF-H Axoplasm from Loligo pealei Particles Prahlad et al., 2000 10 0.5-1.0 0.5-1.0