Table 1.

Forward and reverse primers used to synthesize dsRNA for RNAi in Drosophila S-2 cells (T7 promoter sequence not shown)

Targeted protein Accession number Primers
dVCP (TER94) AE003831 (F) 5′-TAAGACGCTGCTGGCCAA-3′
(R) 5′-CATCGCGGAGTTCTGATTTT-3′
dp47 (CG11139) AE003841 (F) 5′-AGCAAGTGCATCGGCATC-3′
(R) 5′-CGGATCGTCATAGTGACGAA-3′
dUfd1 (CG6233) AE003546 (F) 5′-TCCAATGCTGTTCAAGCTGA-3′
(R) 5′-ATTTCGCCGCCAACAGTT-3′
dUfd2 (CG9934) AE003638 (F) 5′-TTCCTTTCCGTGTCGCTGTT-3′
(R) 5′-AGGCAGGTAGCCCAGATGAT-3′
dNpl4 (CG4673) AE003752 (F) 5′-TGGAGCGCTTCCTCAACTACT-3′
(R) 5′-ATCAGACAATTATCGCGCAC-3′