Table 1.

Mitotic accumulation after 23C-γ-tubulin depletion

Mitotic index (%)
Treatment Incubation time (days) Control Tγ23C
Day 1 5 2.3% (2.0-2.6%) n=10,233 6.5% (6.0-7.0%) n=10,690
7 0.9% (0.8-1.0%) n=10,022 4.1% (3.7-4.5%) n=10,429
9 0.15% (0.1-0.2%) n=10,015 0.35% (0.2-0.5%) n=10,035
Days 1 and 5 7 2.0% (1.7-2.3%) n=10,206 13% (11.6-14.4%) n=2,300
  • The mitotic indices (M.I.) [i.e. the proportion of cells undergoing mitosis divided by total cell number (n)] were determined after chromosome staining (with DAPI) and immunolabelling of the microtubule cytoskeleton (with antibodies against α-tubulin). These quantifications were performed in control cells and in cells treated by RNAi against 23Cγ-tubulin either once (day 1; M.I. quantifications after 5 days, 7 days and 9 days) or twice (days 1 and 5; M.I. quantification after 7 days). All confidence intervals (in parentheses) were calculated for a probability of 95%. Data from a representative experiment are presented. Four additional experiments giving comparable results were performed.