Table 1.

MALDI-TOF mass spectrometry identification of proteins interacting with the C-termini of 5-HT4R splice variants

Accession no. MALDI-TOF Interaction**
Spots* Protein Peptides Coverage (%) PDZ§ Others C-t(a) C-t(b) C-t(e) Functions
1 ARIP 1 (MAGI-2) Q9WVQ1 18 21 6 2 WW, 1 GuK + - - Scaffolding, membrane targeting
2 MPP3 (Dlgh3) O88910 21 45 1 L27, SH3, GuKc + - - Veli-interacting protein
3 Ulip2 (CRMP-2) P47942 19 50 - - + - - Axon growth
4 SNX27 Q8K4V4 18 36 1 PX + - - Intracellular trafficking
5,6,8 NHERF (EBP50) P70441, Q9JJ19, Q9R1A1 40 2 ERM + - - Signalling, cytoskeleton modification
12
7 Guanine aminase Q9R111 13 26 †† - + - - Purine-nucleotide metabolism
9 Veli1 (Lin-7-Bb) Q9Z250 8 36 1 L27 + - - Targeting and trafficking
10 Veli2 (Lin-7B) O88951 9 50 1 L27 + - - Targeting and trafficking
11 Veli3 (Lin-7C) O88952 9 52 1 L27 + - - Targeting and trafficking
12 Peroxiredoxin 5 P99029 11 74 ‡‡ - + - - Intracellular redox signalling
13 nNOS Q9Z0J4 29 25 1 - - - + Signalling
14 CIPP O70471 17 32 4 - - - + Scaffolding
15 SEC23 Q01405 19 34 - - - - + Trafficking from ER to the Golgi apparatus
  • * The numbers correspond to those indicated in Fig. 2.

  • Accession numbers are listed from SWISS-PROT and TrEMBL databanks.

  • When several protein spots were identified as a single protein, the results of the spot for which MALDI-TOF analysis yielded the largest sequence coverage are indicated.

  • § The number of PDZ domains encompassed in the protein sequence or the presence of a putative PDZ ligand.

  • Other protein-protein interaction domains.

  • ** Interaction of the protein with C-terminal peptides.

  • †† C-terminus sequence is SSV.

  • ‡‡ C-terminus sequence is SQL.