Table 1.

Proportions of cells showing different subcellular localizations of Kaiso NLS mutants

Construct Nuclear (%) Cytoplasmic (%) Nuclear and cytoplasmic (%)
β-Gal/GFP 0 72±8.5 28±8.5
β-Gal/NLS/GFP 96±1.4 0 4±1.41
β-Gal/NLSmut/GFP 0 65±15.6 35±15.6
GFP 76.5±9.2 0 23.5±9.2
GFP/Kaiso 99.5±0.7 0 0.5±0.7
GFP/Kaiso/NLSmut 0 63±11.3 37±11.3
  • Each Kaiso deletion mutant was scored blindly as predominantly nuclear, predominantly cytosolic or both nuclear and cytosolic. Each value represents at least two scoring experiments and is expressed as the mean of these experiments ± s.d.