Table 3.

Expression of hepatocyte and cholangiocyte marker genes in colonies

Type IV collagen Laminin
1 2 3 4 5 6 7 8 9 10 Colony number 1 2 3 4 5 6 7 8 9 10
+ + + + + + + + + Albumin + + + + + + + + + +
+ + + + + + G6Pase + + + + + +
+ + CPS + + + + + + +
TAT
+ + + + + + + + + + GS + + + + + + + + +
+
+ + + Cx32 + + + + + +
+ + + + + CK7 + + + + + + + + + +
+ + + + + + + CK19 + + + + + + + + + +
+ + + Integrin β4 + + + + + + + + +
+ + + + + + + + + + Cx43 + + + + + + + + + +
+ + + + + + + + + + HPRT + + + + + + + + + +
  • E14.5 Dlk+ cells were incubated for 21 days. Ten colonies each were selected from among the colonies formed on type IV collagen and laminin. Total RNA was extracted from each colony and used for cDNA synthesis. PCR product was separated on a 1.5% agarose gel and visualized by staining in ethidium bromide. +, PCR product detected; –, PCR product not detected.