Table 1.

Information obtained on the phosphopeptides derived from in vivo labeled vimentin

Peptide number Sequence obtained % Label released on cycle number Total number of cycles
1 pSVSpSSSYR 1 (10%), 4 (70%), D(<10%) 6
2 SVpSpSSSYR 3 (40%), 4 (50%), D (<10%) 6
3 LRpSpSMPGVR 3 (50%), 4 (40%), D (<10%) 6
4 DGQVINEpTpSQHHDDLE* No release, D (>90%) 6
5 SLYSSpSPGGAYVTR 6 (90%), D (<10%) 6
6 TYpSLGpSAL 3 (60%), 6 (20%), D (<10%) 6
7 ETNLEpSLPLVDTHSK 6 (30%), D (>60%) 9
8 ISLPLPNFSpSLNLR No release, D (>90%) 9
  • Phosphorylation sites (indicated in bold) on the basis of the results of the manual Edman degradation.

    Peptides were numbered on the basis of increasing hydrophobicity on C8 HPLC. The sequence obtained for each isolated phosphopeptide is indicated together with the data obtained from the manual Edman degradation. The cycle in which release of label was observed during Edman degradation is shown. D refers to the crosslinked material remaining on the Sequelon membrane discs after completion of the Edman degradation cycles.

  • * The phosphorylation sites of peptide 4 to Thr-457 and Ser-458 were assigned based on the results of Chou et al. (Chou et al., 1996).