Table 2.

Plasmids used in this study

Plasmid Relevant allele Marker Integration site Strains generated
pCDK-FLAG cdk1-1 HygR cdk1 locus TAU17
pKOCDK cdk1Δ::hph HygR cdk1 locus TAU4
pCLB1-VSV clb1-1 HygR clb1 locus UMP19
pKOCLB1 clb1Δ::hph HygR clb1 locus UMP21
pCLB1nar clb1nar CbxR clb1 locus TAU41, UMP25
pRU11-CLB1 Pcrg1clb1-1 CbxR cbx1 locus TAU36-1
pRU12-CLB1 Pcrg1*clb1-1 CbxR cbx1 locus TAU36-2
pRU12-CLB1Δdb1 Pcrg1*clb1Δdb1 CbxR cbx1 locus TAU30
pRU12-CLB1Δdb2 Pcrg1*clb1Δdb2 CbxR cbx1 locus TAU45
pRU12-CLB1Δdb1-2 Pcrg1*clb1Δdb1-2 CbxR cbx1 locus TAU31, TAU57
pCLB2-MYC clb2-1 HygR clb2 locus UMP27
pKOCLB2 clb2Δ::cbx CbxR clb2 locus UMP32
pCLB2nar clb2na CbxR clb2 locus TAU42, UMP26
pRU11-CLB2 Pcrg1clb2-1 CbxR cbx1 locus TAU52-1
pRU12-CLB2 Pcrg1*clb2-1 CbxR cbx1 locus TAU52-2
pRU12-CLB2Δdb Pcrg1*clb2Δdb CbxR cbx1 locus TAU53, TAU58
pCU1-CLB2 Phsp70clb2-1 CbxR cbx1 locus TAU26