Plasmids used in this study
| Plasmid | Relevant allele | Marker | Integration site | Strains generated |
|---|---|---|---|---|
| pCDK-FLAG | cdk1-1 | HygR | cdk1 locus | TAU17 |
| pKOCDK | cdk1Δ::hph | HygR | cdk1 locus | TAU4 |
| pCLB1-VSV | clb1-1 | HygR | clb1 locus | UMP19 |
| pKOCLB1 | clb1Δ::hph | HygR | clb1 locus | UMP21 |
| pCLB1nar | clb1nar | CbxR | clb1 locus | TAU41, UMP25 |
| pRU11-CLB1 | Pcrg1clb1-1 | CbxR | cbx1 locus | TAU36-1 |
| pRU12-CLB1 | Pcrg1*clb1-1 | CbxR | cbx1 locus | TAU36-2 |
| pRU12-CLB1Δdb1 | Pcrg1*clb1Δdb1 | CbxR | cbx1 locus | TAU30 |
| pRU12-CLB1Δdb2 | Pcrg1*clb1Δdb2 | CbxR | cbx1 locus | TAU45 |
| pRU12-CLB1Δdb1-2 | Pcrg1*clb1Δdb1-2 | CbxR | cbx1 locus | TAU31, TAU57 |
| pCLB2-MYC | clb2-1 | HygR | clb2 locus | UMP27 |
| pKOCLB2 | clb2Δ::cbx | CbxR | clb2 locus | UMP32 |
| pCLB2nar | clb2na | CbxR | clb2 locus | TAU42, UMP26 |
| pRU11-CLB2 | Pcrg1clb2-1 | CbxR | cbx1 locus | TAU52-1 |
| pRU12-CLB2 | Pcrg1*clb2-1 | CbxR | cbx1 locus | TAU52-2 |
| pRU12-CLB2Δdb | Pcrg1*clb2Δdb | CbxR | cbx1 locus | TAU53, TAU58 |
| pCU1-CLB2 | Phsp70clb2-1 | CbxR | cbx1 locus | TAU26 |