Table 1.

Mean speed of migration

Genotype Substrate Mean speed μm/min +ve chemotaxis to CSF-1 n
WtCre Glass 0.36±0.006 na 55
Rac1-/- Glass 0.31±0.004 na 70
WtCre Plastic 0.6±0.004 na 158
Rac1-/- Plastic 0.6±0.004 na 143
Wt Glass (Dunn) 0.3±0.003 Yes 64
WtCre Glass (Dunn) 0.35±0.003 Yes 112
Rac1-/- Glass (Dunn) 0.3±0.003 Yes 67
  • To analyse random migration, cells were seeded at a density of 2×104/ml on either glass coverslips or plastic dishes and filmed for 16 hours. Results shown here are the mean of at least three independent experiments. For Dunn chamber analysis, cells were seeded at 2×104/ml, starved of CSF-1 for 8 hours and then filmed in a Dunn chamber (see Materials and Methods) in a gradient of CSF-1 for 20 hours. Results shown here are the mean of at least three independent experiments ±SD. na, not applicable.