Table 1.

Retinal endothelial cell caspase-3 activity as a measure of apoptosis induced in high glucose and high osmolarity is blocked by inhibiting the polyol pathway and by blocking peroxynitrite formation

Caspase-3 activity (fold)
Treatment NG HG 3mG LG
Untreated 4.6±0.45 8.1±0.7* 7.15±0.6* 7.6±0.5*
Zoplorestat 3.6±0.26 4.9±0.29 4.7±0.27 4.6±0.34
Uric acid 3.6±1.34 4.6±0.5 5.1±0.4 4.8±0.7
FeTTPS 3.4±0.31 3.85±0.4 3.7±0.21 ND
L-NAME 3.93±0.3 3.6±0.3 3.5±0.2 ND
SOD 3.8±0.37 5.6±0.6 5.9±0.5 5.8±0.6
  • NG, normal glucose (5 mM); HG, high glucose (25 mM); 3mG, 3-O-methyl glucose (5 mM glucose+20 mM 3mG); LG, L-glucose (5 mM glucose+20 mM L-glucose)

    Zopolrestat (10 μM), an aldose reductase inhibitor; FeTPPS (2.5 μM) and uric acid (1 mM), peroxynitrite scavengers; L-NAME (0.5 mM), a NOS inhibitor; SOD (100 U/ml) superoxide dismutase.

  • * P<0.05 compared with NG; P<0.05 compared with untreated HG, 3mG or LG.