Table 2.

High glucose- and high osmolarity-induced apoptosis of retinal endothelial cells is blocked by inhibiting peroxynitrite formation

% Apoptotic cells
Treatment NG HG 3mG LG
Untreated 16.8±1.5 31.4±1.9* 28.1±2.5* 25.8±2.1*
Uric acid 15.7±1.3 19.5±1.7 20.2±1.7 19.7±1.7
L-NAME 14.6±1.3 20.1±1.9 19.65±1.8 17.6±1.5
SOD 15.7±1.4 26.3±2.3 22.9±2.1 21.5±1.7
  • Percentage of apoptotic cells were calculated by counting 10 fields, 200 cells each in at least three independent cultures.

    NG, normal glucose (5 mM); HG, high glucose (25 mM); 3mG, 3-O-methyl glucose (5 mM glucose+20 mM 3mG); LG, L-glucose (5 mM glucose+20 mM L-glucose)

    Uric acid (1 mM) a peroxynitrite scavenger; L-NAME (0.5 mM), a NOS inhibitor; SOD (100 U/ml), superoxide dismutase.

  • * P <0.05 compared with NG; P <0.05 compared with untreated HG, 3mG or LG.