Table 2.

Incorporation of radioactivity from [14C]glucose into cell wall polysaccharides during bgs4+ shut-off of S. pombe wild-type and mutant bgs4Δ p81X-bgs4+ strains

% Incorporation of [14C]glucose (mean±s.d.)
Growth Strain Thiamine* Cell wall α-Glucan β-Glucan Galactomannan
EMM bgs4Δ p81X-bgs4+ - (on) 33.5±0.8 (100) 10.1±0.7 (30.1) 19.4±0.2 (57.9) 4.0±0.3 (12.0)
bgs4Δ p81X-bgs4+ + (off) 43.7±0.1 (100) 19.6±0.5 (44.9) 20.0±0.6 (45.9) 4.1±0.2 (9.2)
EMM+S bgs4Δ p81X-bgs4+ - (on) 29.5±0.2 (100) 8.2±0.3 (27.9) 17.1±0.2 (57.8) 4.2±0.1 (14.3)
bgs4Δ p81X-bgs4+ + (off) 36.3±0.1 (100) 16.5±0.1 (45.5) 15.3±0.1 (42.2) 4.5±0.1 (12.3)
  • * Induction of p81X-bgs4+ maintains the bgs4Δ cells with wild-type GS activity and morphology. bgs4+-repressed cell cultures were grown for 16 hours in the presence of thiamine. [14C]glucose was added 4 hours before harvesting.

  • Percentage incorporation of [14C]glucose=cpm incorporated per fraction × 100/total cpm incorporated. Values are the means and standard deviations (s.d.) calculated from three independent experiments.

  • Values in parentheses are percentages of the corresponding polysaccharide in the cell wall composition.