Table 3.

Incorporation of radioactivity from [14C]glucose into cell wall polysaccharides during bgs4+ overexpression of S. pombe wild-type and mutant bgs4Δ p3X-bgs4+ strains

% Incorporation of [14C]glucose (mean±s.d.)
Time (hours) Strain Thiamine* Cell wall α-Glucan β-Glucan Galactomannan
15 bgs4Δ p3X-bgs4+ + 31.8±0.7 (100) 14.2±0.2 (44.6) 15.5±0.5 (48.9) 2.1±0.1 (6.5)
bgs4Δ p3X-bgs4+ -(OE) 31.4±0.1 (100) 15.2±0.6 (48.6) 13.5±0.5 (42.8) 2.7±0.2 (8.6)
30§ bgs4Δ p3X-bgs4+ + 32.1±0.7 (100) 13.4±0.6 (41.8) 15.7±0.7 (48.9) 3.0±0.2 (9.3)
bgs4Δ p3X-bgs4+ -(OE) 37.4±0.8 (100) 20.7±1.5 (55.4) 14.5±1.4 (38.8) 2.2±0.4 (5.8)
  • OE, overexpressed.

  • * Repression of p3X-bgs4+ maintains the bgs4Δ cells with wild-type GS activity and morphology.

  • Values were calculated from three independent experiments, as described in Table 2.

  • [14C]glucose was added to the cell cultures 6 hours before harvesting.

  • § [14C]glucose was added to the cell cultures 7 hours (control cells, +thiamine) or 21 hours (bgs4+-overexpressing cells, -thiamine) before harvesting.