Table 1.

Half-time of recovery values for GFP-MBNL1

Cell type Photobleached region t½ (seconds) of recovery ± 2 × s.e.m. P value (Student's t-test) Immobile fraction ± 2 × s.e.m. P value (Student's t-test)
COSM6 960 CTG repeats CUG foci (n=42) 3.7±0.3 0.6427 37.2±3.7 <0.0001
COSM6 960 CTG repeats CUG nucleoplasm (n=42) 3.1±0.3 0.0867 2.5±1.6 0.2428
COSM6 960 CAG repeats CAG foci (n=34) 3.6±0.4 33.2±4.4 <0.0001
COSM6 960 CAG repeats CAG nucleoplasm (n=34) 3.5±0.3 3.9±2.7 0.7358
COSM6 GFP-MBNL1 alone (n=45) 3.3±0.1 0.1137 3.5±0.9
GM03132 DM patient fibroblast (2000 CTG) CUG foci (n=37) 3.5±0.4 0.1890 13.9±2.8 0.3221
GM03132 DM patient fibroblast (2000 CTG) CUG nucleoplasm (n=37) 3.5±0.2 0.0066 2.1±1.6 0.7644
GM03987 DM patient fibroblast (500 CTG) CUG foci (n=31) 3.1±0.3 16.2±3.6
GM03987 DM patient fibroblast (500 CTG) CUG nucleoplasm (n=31) 3.0±0.3 1.8±2.0
  • P values for the half-times of GFP-MBNL1 recovery for foci and nucleoplasm were calculated using an unpaired Student's t-test comparing CUG and CAG foci or nucleoplasm of CUG, CAG and GFP-MBNL1 alone. For t½, P values were calculated using an unpaired Student's t-test comparing the half-times of recovery for CUG vs CAG foci, and CUG vs CAG nucleoplasm. For the immobile fraction, P values were calculated using an unpaired Student's t-test comparing the extent of recovery (relative to pre-bleach intensity) in foci vs nucleoplasm.