Cell type | Photobleached region | t_{½} (seconds) of recovery ± 2 × s.e.m. | P value (Student's t-test) | Immobile fraction ± 2 × s.e.m. | P value (Student's t-test) |
---|---|---|---|---|---|
COSM6 960 CTG repeats | CUG foci (n=42) | 3.7±0.3 | 0.6427 | 37.2±3.7 | <0.0001 |
COSM6 960 CTG repeats | CUG nucleoplasm (n=42) | 3.1±0.3 | 0.0867 | 2.5±1.6 | 0.2428 |
COSM6 960 CAG repeats | CAG foci (n=34) | 3.6±0.4 | 33.2±4.4 | <0.0001 | |
COSM6 960 CAG repeats | CAG nucleoplasm (n=34) | 3.5±0.3 | 3.9±2.7 | 0.7358 | |
COSM6 | GFP-MBNL1 alone (n=45) | 3.3±0.1 | 0.1137 | 3.5±0.9 | |
GM03132 DM patient fibroblast (2000 CTG) | CUG foci (n=37) | 3.5±0.4 | 0.1890 | 13.9±2.8 | 0.3221 |
GM03132 DM patient fibroblast (2000 CTG) | CUG nucleoplasm (n=37) | 3.5±0.2 | 0.0066 | 2.1±1.6 | 0.7644 |
GM03987 DM patient fibroblast (500 CTG) | CUG foci (n=31) | 3.1±0.3 | 16.2±3.6 | ||
GM03987 DM patient fibroblast (500 CTG) | CUG nucleoplasm (n=31) | 3.0±0.3 | 1.8±2.0 |
P values for the half-times of GFP-MBNL1 recovery for foci and nucleoplasm were calculated using an unpaired Student's t-test comparing CUG and CAG foci or nucleoplasm of CUG, CAG and GFP-MBNL1 alone. For t_{½}, P values were calculated using an unpaired Student's t-test comparing the half-times of recovery for CUG vs CAG foci, and CUG vs CAG nucleoplasm. For the immobile fraction, P values were calculated using an unpaired Student's t-test comparing the extent of recovery (relative to pre-bleach intensity) in foci vs nucleoplasm.