Table 3.

Motility-related calpain substrates

Calpain substrate In vitro Cell culture In vitro isoforms* Cell culture isoforms References
α-actinin No Yes - ND Goll et al., 1991; Selliah et al., 1996
β integrins Yes Yes Capn2 ND Du et al., 1995; Pfaff et al., 1999
β-catenin Yes Yes Capn1 Capn2 Rios-Doria et al., 2004
Cadherins Yes Yes Capn1, 2 ND Covault et al., 1991; Rios-Doria et al., 2003
Cortactin Yes Yes Capn1, 2 Capn2 Huang et al., 1997
EGFR Yes Yes ND ND Gates and King, 1983; King and Gates, 1985; Stoscheck et al., 1988
FAK Yes Yes Capn1, 2 Capn2 Carragher et al., 1999; Cooray et al., 1996; Franco et al., 2004a
Filamin Yes Yes Capn1, 2, 3 Capn2, 3 Huff-Lonergan et al., 1996; Kwak et al., 1993; Verhallen et al., 1987
MARCKS Yes Yes Capn2 Capn1, 2 Dedieu et al., 2003; Dulong et al., 2004a
MLCK Yes Yes ND ND Kambayashi et al., 1986; Kosaki et al., 1983
Paxillin Yes Yes Capn1, 2 Capn2 Carragher et al., 1999; Franco et al., 2004a
PTP-1B Yes Yes Capn1 ND Frangioni et al., 1993; Schoenwaelder et al., 1997
RhoA Yes Yes Capn1 ND Kulkarni et al., 2002
Spectrin Yes Yes Capn1, 2 Capn2 Croall et al., 1986; Fox et al., 1987; Franco et al., 2004a
Src ND Yes ND ND Oda et al., 1993
Talin 1 Yes Yes Capn1, 2 Capn2 Carragher et al., 1999; Franco et al., 2004a
Vinculin ND Yes ND ND Serrano and Devine, 2004
  • * Proteolysis by unlisted isoforms has not been determined. ND, not determined.