Table 1.

Molecular analyses of cholesterol-depleted and recovered CV

Sample Fusion EC50 (μM) Fusion extent (%) Fusion kinetics (%/second) Cholesterol/CV (amol) CH:PL
Control 28.4±2.8 100.0** 92.4±6.1 70.6±3.3 0.602±0.034
Cholesterol depleted§ 50.0±6.6* 78.6±4.5* 43.5±4.1* 49.5±2.0* 0.411±0.021*
Cholesterol recovered 28.3±8.6 95.2±7.7 85.9±9.7 74.8±4.9 0.637±0.045
  • * Significant difference from control (P<0.05).

  • Initial rate in response to 96±11 μM [Ca2+]free.

  • Molar cholesterol:phospholipid ratio.

  • § Treated with 2 mM mβcd for 30 minutes at 25°C.

  • Treated with 2 mM hpβcd-cholesterol for 30 minutes at 25°C after treatment with 2 mM mβcd.

  • ** 100% fusion by definition.