Table 1.

Strains used in this study

Strain Genotype Source
CD16-1 h+/h ade6-M210/ade6-M216 cyhl/+ +/lys5-391 C. Shimoda (Osaka City University, Osaka, Japan)
CD16-5 h/h ade6-M210/ade6-M216 cyhl/+ +/lys5-391 C. Shimoda
ST194 h/h ade6-M216/210 leu1-32/leu1-32 mcp6::[mcp6-GFP-3′UTR-Lys3+]/mcp6:: [mcp6-GFP-3′UTR-Lys3+] pat1-114/pat1-114
ST142 h90 ade6-M216 leu1-32 ura4-D18 mcp6::[mcp6-GFP-3′UTR-Lys3+] dsRed-Sad1 [::LEU2]
JZ670 h/h ade6-M210/ade6-M216 leu1-32/leu1-32 pat1-114/pat1-114 M. Yamamoto (University of Tokyo, Tokyo, Japan)
TT405 h/h ade6-M216/210 leu1-32/leu1-32 ura4-D18/ura4-D18 mcp6::ura4+/mcp6::ura4+ pat1-114/pat1-114
CT026-1* h90 leu1-32 ura4-D18 TB19::GFP-lys1+ Y. Hiraoka (Kamsai Advanced Research Center, Kobe, Japan)
ST193* h90 leu1-32 ura4-D18 mcp6::ura4+ TB19::GFP-lys1+
AY174-7B h90 leu1-32 ura4-D18 ade6-M210 his7::lacI-GFP-NLS-his7+ lys1::lacOr-lys1+ K. Nabeshima (Stanford University, Stanford, CA) and A. Yamamoto (Kamsai Advanced Research Center, Kobe, Japan)
ST197 h90 leu1-32 ura4-D18 mcp6::ura4+ his7::lacI-GFP-NLS-his7+ lys1::lacOr-lys1+
TT8-1 h ura4+
NP32-2A h+ leu1-32 his2 ura4-D18 Nabeshiwa et al., 2001
TT232-1 h+ his2 leu1-32 ura4-D18 lys3 cdc12
MS105-1B h ade6-M26 ura4-D18 Shimada et al., 2002
MS111w1 h+ ade6-469 ura4-D18 leu1-32 his2 Shimada et al., 2002
GP1123 h+ ade6-D1 ura4-D18 leu1-32 zzz7::[ade6-469 ura4+] G. Smith
TT398 h ura4-D18 mcp6::ura4+
TT399 h+ his2 leu1-32 ura4-D18 mcp6::ura4+
TT411 h ura4-D18 mcp6::ura4+ cdc12 lys3
TT400 h ade6-M26 ura4-D18 mcp6::ura4+
TT401 h+ ade6-469 his2 leu1-32 ura4-D18 mcp6::ura4+
TT1014 h+ ade6-D1 leu1-32 ura4-D18 mcp6::ura4+ zzz7::[ade6-469 ura4+]
TP4-5A h ade6-M210 ura4-D18 leu1-32 C. Shimoda
TP4-1D h+ ade6-M216 his2 leu1-32 ura4-D18 C. Shimoda
TT397-5A h ade6-M210 leu1-32 ura4-D18 mcp6::ura4+
TT397-1D h+ ade6-M216 his2 leu1-32 ura4-D18 mcp6::ura4+
CRL790 h90 ade6-216 leu1-32 ura4-D18 lys1 dsRed-Sad1 [::LEU2] Y. Hiraoka
ST148 h90 ade6-M216 leu1-32 ura4-D18 mcp6::ura4+ dsRed-Sad1[::LEU2]
ST176 h90 leu1-32 ura4-D18(or+) dsRed-sad1[::LEU2] spo15-GFP::LEU2
ST171-1 h90 ade6-M210 leu1-32 ura4-D18(or+) mcp6::ura4+ dsRed-sad1[::LEU2] spo15-GFP::LEU2
ST191-1 h90 leu1-32(or+) dsRed-sad1[::LEU2] kms1::GFP::Kanr
ST172-1 h90 ura4-D18(or+) mcp6::ura4+dsRed-sad1[::LEU2] kms1-GFP::Kanr
ST178 h90 ade6-M216 leu1-32(or+) ura4-D18(or+) dsRed-sad1[::LEU2] Taz1::GFP::Kanr
ST173 h90 ade-M216 ura4-D18(or +) mcp6::ura4+ dsRed-sad1[::LEU2] Taz1::GFP::Kanr
ST179-1 h90 leu1-32 ura4-D18 dsRed-sad1[::LEU2] swi6+::GFP-leu2
ST174 h90 leu1-32 ura4-D18 mcp6::ura4+ dsRed-sad1[::LEU2] swi6+::GFP:leu2
YY105 h90 leu1-32 ura4-D18 lys1::nmt1pGFP-alpha2tubulin Y. Hiraoka
ST146 h90 leu1-32 ura4-D18 mcp6::ura4+ lys1::nmt1pGFP-alpha2tublin
ST134 h90 ade6-M210 leu1-32 ura4-D18 mcp6::[mcp6-GFP-3′UTR-Lys3+]
ST196-1 h90 ade6-M210 leu1-32 dhc1-d3[LEU2] mcp6::[mcp6-GFP-3′UTR-Lys3+] dsRed-Sad1[::LEU2]
ST200 h90 ade6-M210 leu1-32 ura4-D18 taz1::ura4+ mcp6::[mcp6-GFP-3′UTR-Lys3+]
  • * TB19 signifies the N-terminal portion of DNA polymerase α (Ding et al., 2000).

  • Unattributed strains were constructed for this study.