Table 1.

Primers

A. Real time PCR primers
Genes Positions (bp) Sequences
Apolipoprotein J 937-959 5′-gga tga agg acc agt gtg aca ag-3′
1032-1050 5′-cag cga cct gga ggg att c-3′
Fibronectin 5023-5041 5′-tgt ggt tgc ctt gca cga t-3′
5111-5131 5′-gct tgt ggg tgt gac ctg agt-3′
Osteonectin 868-889 5′-gag acc tgt gac ctg gac aat g-3′
957-982 5′-gga agg agt gga ttt aga tca caa ga-3′
SM22 511-530 5′-cgt gga gat ccc aac tgg tt-3′
586-606 5′-aag gcc aat gac atg ctt tcc-3′
p21WAF-1 495-515 5′-ctg gag act ctc agg gtc gaa-3′
599-617 5′-cca gga ctg cag gct tcc t-3′
p53 1209-1229 5′-aag aaacca ctg gat gga gaa-3′
1263-1283 5′-cag ctc tcg gaa cat ctc gaa-3′
c-fos 249-268 5′-tca ccc gca gac tcc ttc tc-3′
323-343 5′-gtg gga atg aag ttg gca ctg-3′
c-jun 2016-2033 5′-gga tca agg cgg aga gga a-3′
2088-2102 5′-tcc agc cgg gcg att-3′
TGF-β1 1788-1808 5′-agg gct acc atg cca act tct-3′
1869-1889 5′-ccg ggt tat gct ggt tgt aca-3′
TGF-β2 1067-1083 5′-acc aac cgg cgg aag aa-3′
1153-1176 5′-cac cct aga tcc ctc ttg aaa tca-3′
TGF-β3 1288-1305 5′-agg ccc ttg ccc ata cct-3′
1349-1372 5′-aga tgc ttc agg gtt cag agt gtt-3′
GAPDH 942-963 5′-acc cac tcc tcc acc ttt gac-3′
1033-1053 5′-gtc cac cac cct gtt gct gta-3′
B. Primers used for the detection of the common 4977 bp mitochondrial DNA deletion
Primers Positions (bp) Sequences
H1 219-238 5′-atg ctt gta gga cat aat aa-3′
L1 447-466 5′-agt ggg agg gga aaa taa ta-3′
H2 8150-8169 5′-ccg ggg gta tac tac ggt ca-3′
L2 13631-13650 5′-ggg gaa gcg agg ttg acc tg-3′
H3 8197-8215 5′-cag ttt cat gcc cat cgt c-3′
L3 13560-13578 5′-gat gag agt aat aga tag g-3′
C. Primers used to construct the different apo J retroviral expression vectors
Primers Sequences
APOJ31F 5′-cgc gga tcc gcg gcg acc atg atg aag act ctg ctg ctg tt-3′
APOJ31R 5′-tgc tct aga gcc ctc ctc ccg gtg ctt ttt gcg-3′
APOJPNF 5′-g ggt taa ccc gcc acc atg atg aag act ctg ctg ctg tt-3′
APOJPNR 5′-c ggg atc ccg gcg ggt tta aac tca atg gtg-3′
TRAPOJR 5′-c ggg atc ccg tca ctc ctc ccg gtg ctt ttt gc-3′