Table 4.

Summary of conditions observed in time-lapse studies of primary spermatocytes in both control and hemizygous vibrator mutants

Condition Wild type (Oregon-R)*vibS110416 / Df(3R)Dl-Bx12
Anaphase onset to furrow initiation if cleavage successful (minutes) 9.8±0.5 (n=6) 9.7±0.9 (n=3)
Anaphase onset to furrow initiation if cleavage unsuccessful (minutes) 14.0±1.7 (n=4)
Rate of furrow ingression (μm/minute) 1.9±0.15 (n=9) 1.4±0.07 (n=7)
Furrow initiation to cleavage (minutes) 22.8±1.0 (n=8) 44.3±5.2 (n=3)
Furrow initiation to rapid membrane regression (minutes) 38±9.5 (n=4)
Central spindle diameter (μm) if cleavage successful 2.3±0.05 (n=8) 2.4±0.06 (n=3)
Central spindle diameter (μm) if cleavage unsuccessful 4.5±1.0 (n=4)
  • Conditions are given as average ± s.e.; n, number of cells

  • * Cytokinesis was not observed in one of the 9 wild-type cells filmed. This appears to be due to the flattening of cells and has been previously described (Inoue et al., 2004)

  • Cleavage was unsuccessful in four of the seven vibrator cells filmed

  • In 3 of 9 wild-type cells, filming commenced after initiation of anaphase and so this interval could not be measured