Table 1.

Quantification of GFP-LC3, Rab7 and mAtg9 co-localization

GFP-LC3 with Rab7 Rab7 with GFP-LC3 GFP-LC3 with mAtg9 mAtg9 with GFP-LC3 Rab7 with mAtg9 mAtg9 with Rab7
51.2±9.4% 6.7±1.2% 45.9±0.6% 7.4±0.7% 26.8±3.7% 32.3±5.0%
  • Images were analysed using the co-localization function of the Zeiss LSM-510 software. Quantification was performed for each cell, using a region of interest, and data are the mean values of 20 cells obtained from two independent experiments, %±s.e.m.