Table 1.

Quantification of emigrated neurosphere cells 24 hours after transplantation

Treatment n Emigration/No emigration P*
Exp. 1 (Chick) BMP-2 10 7 / 3 0.003
No BMP-2 9 0 / 9
Exp. 2 (Chick) BMP-2 8 6 / 2 0.007
No BMP-2 7 0 / 7
Exp. 3 (Chick) BMP-2 4 2 / 2 n.d.
No BMP-2 4 0 / 4
Exp. 4 (Quail) BMP-2 4 4 / 0 n.d.
No BMP-2 1 0 / 1
Total 47 19 / 28
  • For statistical evaluation, transplanted neurospheres were rated for `emigration' or `no emigration'. Only neurospheres with emigration of single cells beyond the lumen of the neural tube were classified as `emigration'. The correlation between BMP-2 treatment and emigration was statistically significant (P<0.01) in experiments 1 and 2. Owing to the low number of observations, experiments 3 and 4 could not be evaluated statistically, although these results were comparable to experiments 1 and 2. *Fisher's Exact Test, two-tailed; n, number of embryos.