Table 1.

Components found in PMA-induced podosomes in HUVECs

Component Podosome localisation Staining used
F-actin core Phalloidin
Cortactin core Anti-cortactin Ab (Upstate Biotechnogy)
Gelsolin core Ab from C. Chaponnier (Geneva)
Vinculin ring hVIN-1 Ab (Sigma)
α-Actinin core Anti-α-actinin Ab (Sigma)
Paxillin ring Anti-paxillin Ab (Transduction Laboratories)
Dynamin-2 core Ab from M. McNiven (Rochester)
Arp2/3 core Ab from M. Welch (Berkeley)
N-WASP core Ab from M. Way (London)
WIP core Myc-tag construct from D. Stewart (Bethesda)
Phosphotyrosine core + ring 4G10 monoclonal Ab
Phospho-ser/thr core + ring Monoclonal Ab (Transduction Laboratories)
PI3K ring Anti-p85α Ab (monoclonal U5)
Cdc42 core Anti-Cdc42 Ab (Transduction Laboratories)
αvβ3 Integrin ring Anti-αvβ3 Ab (Chemicon)
MMP-2 core + ring Anti-MMP2 (Interchim)
MT1-MMP core Anti-MT1-MMP Ab (Oncogene)
  • Ab, antibody.