Table 3.

Plasmids used in this study

Name Yeast markers Cloned gene Source
pRS303-MPC54-RFP HIS3, Integration RFP fusion to MPC54 This study
pRS306-MPC54-RFP URA3, Integration RFP fusion to MPC54 This study
pRS424-DTR1-GFP TRP1, 2μ GFP fusion to DTR1 This study
pRS424-DTR1-Δ3-51SPO20 TRP1, 2μ DTR1 fusion to SPO20Δ3-51 This study
pRS424-DTR1-Δ1-370SEC9 TRP1, 2μ DTR1 fusion to SEC9Δ1-370 This study
pRS424-GFP-SPO14K→H TRP1, 2μ GFP fusion to SPO14 K→H mutant This study
pRS424-G20 TRP1, 2μ GFP fusion to SPO20 nucleotides 151-273 This study
pRS426-G20 URA3, 2μ GFP fusion to SPO20 nucleotides 151-273 Nakanishi et al., 2004
pRS426-SPO20pr-Δ3-51SPO20 URA3, 2μ SPO20Δ3-51 Nakanishi et al., 2004