Table 1.

Yeast two-hybrid interaction assays: αT-catenin specifically interacts with plakophilin-1, -2 and -3

Number Bait plasmid Prey plasmid Interaction
A
   1 pGBT9-α-catulin pGADT7-hPKP3
   2 pGBT9-αE-catenin pGADT7-hPKP3
   3 pGBT9-αN-catenin pGADT7-hPKP3
   4 pGBT9-αT-catenin pGADT7-hPKP3 +
   5 pGBKT7-αT-catenin pGADT7-hPKP3 +
   6 pGBKT7-hPKP3 pGADT7-αT-catenin +
   7 pGBKT7-hPKP3 pGAD424-αE-catenin
   8 pGBKT7-hPKP3 pGAD424-αN-catenin
B
   9 peGBKT7-hαT-catenin pGAD424-β-catenin(N-term) +
   10 peGBKT7-hαT-catenin pGADT7-hPKP1 +
   11 pAS2-hPKP2 pGADT7-αT-catenin +
   12 pAS2-hPKP2 pGAD424-αE-catenin
C
   13 pGBKT7-p0071 head ΔC2 pGADT7-αT-catenin
   14 pGBKT7-p0071 head ΔN1 pGADT7-αT-catenin
   15 pdGBKT7-hδ-catenin short pGADT7-αT-catenin
   16 peGBKT7-hαT-catenin pdGADT7-hδ-catenin short
   17 pGBKT7-p120ctn-3AC pGADT7-αT-catenin
   18 pGBKT7-mARVCF CM pGADT7-αT-catenin