Table 1.

Lysosomes (Ly) and post lysosomes (PL) in WT and lvsB mutant cells *

Diameter (μm) Area (μm2) Number of vacuoles/confocal image Total area/confocal image (μm2) Total area (Ly+PL) (μm2)
Cells Ly PL Ly PL Ly PL Ly PL
WT 1.16±0.01 1.75±0.07 4.65±0.13 10.32±0.93 11.44±0.26 2.61±0.07 53.21±1.37 26.84±1.78 80.05±2.03
lvsB 1.47±0.04 1.88±0.08 8.01±0.50 11.84±0.99 8.19±0.34 1.24±0.05 65.26±1.36 14.56±0.65 79.82±1.98
  • * Analysis was performed as described in the Materials and Methods. It was repeated on three different sets of images. Each set comprised at least 17 images of WT and lvsB mutant cells. The values are means of these three experiments (± s.e.m.)

  • To calculate the surface area of lysosomes and post lysosomes they were assumed to be spherical (Surface area=πd2)