The secondary structural contents of WT and domain swap PF14 fragments determined by CD
PF 14 | Alpha helix | Beta strand | Turns | Unordered |
---|---|---|---|---|
WT | 4 | 40 | 22 | 34 |
3-4-5-6 | 4 | 40 | 23 | 33 |
3-20-21-6 | 4 | 44 | 21 | 31 |
19-4-5-6 | 4 | 42 | 20 | 34 |
Deconvolution of the raw data for each fragment was performed using CDSSTR software programme (Sreerama and Woody, 2000), which is available on the online server dichroweb (Whitmore and Wallace, 2004) (http://www.cryst.bbk.ac.uk/cdweb/html/home.html). The PF14 fragments all had similar secondary structures as predicted by their domain structures