Table 1.

Effect of different mutations on PO activation in the clot

Mutant Melanization Reference
da-GAL4>UAS-spn27A
lz-GAL4>UAS-spn27A
Bc Scherfer et al., 2004
lz Bidla et al., 2005
lz-GAL4>UAS-CG3066-IR +
Hmlf03374 +++
GNBP-1 +++
PGRP-SA +++
PGRP-LC +++
PGRP-LE +++
relish +++
spz2 +++
dTAK1 +++
Act5C-GAL4>UAS-bsk-IR
lz-GAL4>UAS-bsk-IR ++
Act5C-GAL4>UAS-hep-IR
he-GAL4>UAS-RhoA.DA
egr1/egr1 +
egr3/egr3 ++
egr1/def +/−
he-GAL4>UAS-p35 +
  • Hemolymph from mutant animals was prepared and analyzed for melanization of the clot. Loss of melanization is indicated by − and different grades of melanization by: +/−, very weak; +, weak; ++, intermediate and +++, normal. Partial melanization with knockout were lz-Gal4 was used may be due to the early transient expression of lz. Melanization of clots from egr1 homozygous mutants was stronger than in transheterozygotes with a deletion uncovering the same region compatible with the idea that egr1 shows some residual activity (see also Fig. 4 for further analysis of egr mutants).