Table 1.

Dynamics of PML NB components in the nucleoplasm and at NBs

Protein D [μm2 second–1] α kon [second–1] koff [second–1] kin [second–1] kout [second–1] Rt [second] bndout bndin
PML I 1.85 0.47 0.0698 0.0051 0.0017 0.0045 272.3 0.7216 0.2784
PML II 1.03 0.44 0.0510 0.0051 0.0013 0.0015 372.7 0.5263 0.4737
PML III 1.63 0.53 0.0421 0.0026 <0.0001 <0.0001 450.9 0.8663 0.1337
PML IV 1.04 0.50 0.0420 0.0034 0.0007 0.0012 452.9 0.6416 0.3584
PML V 2.79 0.57 0.0065 0.0004 <0.0001 <0.0001 2900.9 0.7232 0.2768
PML VI 1.49 0.51 0.0358 0.0027 <0.0001 <0.0001 530.6 0.6981 0.3019
PML IV-2K 1.19 0.55 0.0860 0.2709 0.0118 0.0205 5.8 0.6348 0.3651
PML IV-3K 2.23 0.61 0.0657 0.0293 0.0046 0.0001 1507.1 0.0227 0.9773
PML-RARα 0.93 0.45 0.0039 0.0018 0.0024 0.0070 2297.0 0.2413 0.7587
SP100 1.23 0.62 0.3940 0.0578 0.0103 0.0058 48.2 0.3585 0.6415
SP100K297R 0.84 0.58 0.8517 0.0978 0.0003 0.0003 22.3 0.4585 0.5415
HIPK2 2.60 0.66 0.2722 0.6216 0.0744 0.0127 11.0 0.8540 0.1460
HIPK2mut 0.66 0.50 0.4242 0.8173 0.0730 0.0788 2.4 0.5190 0.4810
DAXX 0.95 0.60 0.7552 0.4683 0.0028 0.0116 2.6 0.8064 0.1936
BLM 3.10 0.61 0.9700 0.2765 0.0009 0.0062 4.1 0.8769 0.1231
nls-GFP 9.50 0.73 n.a. n.a. n.a. n.a. n.a. n.a. n.a.
  • The diffusion-binding model was used to extract kinetics data from the FRAP experiments of the indicated PML NB components after fitting. The diffusion coefficients (D) and the anomaly parameters (α) were determined by FCS. kon, association rate at the surface of the nuclear body; koff, dissociation rate at the surface of the nuclear body; kin, penetration rate into the nuclear body; kout, penetration rate out of the core of the nuclear body; Rt, mean residence time at nuclear bodies; bndout, fraction of molecules residing at the surface of the nuclear body; bndin, fraction of molecules residing in the core of the nuclear body. FCS data obtained for GFP tagged with a nuclear localization signal (nls-GFP) are also shown (n.a., not applicable)