Table 1.

Characterization of anaphase-B spindle elongation in cells expressing kinesin-5 mutants and Ase1-GPF

Fast phase Slow phase Pause
Genotype Ratea,c μm/minute Spindle lengthc μm Ratea,c μm/minute Spindle lengthb,c μm % occurrence (n/total)
WT 0.85±0.03 4.86±0.24 0.25±0.02 7.35±0.48 0
(8) (10) (9) (10) (0/10)
kip1Δ 0.74±0.04*,d 4.85±0.26 0.20±0.01*,d 8.18±0.55 0
(10) (11) (10) (5) (0/11)
cin8-FA 0.84±0.06 4.78±0.18 0.19±0.01*,d 7.55±0.37 50
(8) (12) (6) (4) (6/12)
cin8-3 0.77±0.05 6.40±0.44**,d 0.19±0.02 14
(6) (7) (7) (1/7)
kip1Δ cin8-FA 0.50±0.04***,d 3.89±0.23**,d 0.17±0.01**,d 7.41±1.15 76
(11) (14) (7) (4) (13/17)
kip1Δ cin8-3 0.78±0.06 5.16±0.17 0.21±0.02 8.21±0.58 42
(9) (19) (16) (11) (8/19)
Ase1-GFP 0.73±0.05 5.8±0.24 0.21±0.02 7.38±0.24 0
(11) (12) (14) (8) (0/12)
kip1Δ Ase1-GFP 0.62±0.04*,e 4.80±0.19 0.17±0.01 6.9±0.3*,e 62
(13) (13) (13) (13) (8/13)
kip1Δ Ase1-GFP (pASE1)f 0.62±0.10 4.54±1.09 0.24±0.05 8.31±0.59 0
(5) (7) (10) (4) (0/7)
  • a Rates of spindle elongation determined for those cases in which there were at least four time points on the slope or the plot of SPB distance vs time

  • b Determined for those cells in which, at the end of anaphase, spindle disassembly was observed (Fig. 1Cb) and/or final spindle length remained constant for at least 5 minutes (Fig. 1Ca)

  • c Numbers in parentheses indicate the number of averaged experiments

  • d Significance compared with WT cells

  • e Significance compared with kip1Δ cells

  • f Extra copy of ASE1 is expressed form a centromeric plasmid

  • * P<0.05

  • ** P<0.01

  • *** P<0.001