Table 2.

Characterization of anaphase-B spindle elongation in slk19Δ and spo12Δ cells

Fast phase Slow phase Pause
Genotype Ratea,c μm/minute Spindle lengthc μm Ratea,c μm/minute Final spindle lengthb,c μm % occurrence (n/total)
WT 0.85±0.03 4.86±0.24 0.25±0.02 7.35±0.48 0
(8) (10) (9) (10) (0/10)
slk19Δd 0.92±0.02*,e 5.31±0.30 0.31±0.03 7.49±0.41 0
(8) (8) (8) (6) (0/8)
spo12Δ 0.67±0.07*,e 4.53±0.40 0.28±0.04 6.80±0.22 0
(6) (8) (8) (5) (0/8)
kip1Δ cin8-FA 0.50±0.04 3.89±0.23 0.17±0.01 7.41±1.15 76
(11) (14) (7) (4) (13/17)
kip1Δ cin8-FA slk19Δ 0.69±0.03***,f 5.70±0.25***,f 0.30±0.02**,f 7.69±0.17 0
(15) (25) (19) (25) (0/25)
  • a Rates of spindle elongation determined for those cases in which there were at least four time points on the slope or the plot of SPB distance vs time

  • b Determined for those cells in which, at the end of anaphase, spindle disassembly was observed (Fig. 1Cb) and/or final spindle length remained constant for at least 5 minute (Fig. 1Ca)

  • c Numbers in parentheses indicate the number of averaged experiments

  • d Averages calculated on cells with bi-phasic spindle elongation

  • e Significance compared with wild-type (WT) cells

  • f Significance compared with kip1Δ cin8-FA cells

  • * P<0.05

  • ** P<0.01

  • *** P<0.001