Table 3.

Characterization of anaphase-B spindle elongation in kip1Δ cin8-FA cells overexpressing Cdc20

Fast phase Slow phase Pause
Plasmid and medium Ratea,c μm/minute Spindle lengthc μm Ratea,c μm/minute Spindle lengthb,c μm % occurrence (n/total)
Vector 0.52±0.04 2.97±0.32 0.26±0.02 6.81±0.65 83
(5) (5) (6) (6) (5/6)
Vector (– methionine) 0.53±0.05 3.12±0.36 0.20±0.02 5.98±0.76 63
(8) (8) (8) (7) (5/8)
PMET→CDC20 0.49±0.03 3.78±0.69 0.24±0.02 6.88±0.35 90
(10) (10) (9) (9) (9/10)
PMET→CDC20 (– methionine) 0.38±0.03 3.22±0.32 0.12±0.01 4.83±0.64 0
(6) (6) (9) (5) (0/9)
  • a Rates of spindle elongation are determined for those cases in which there were at least four time points on the slope or the plot of SPB distance vs time

  • b Determined for those cells in which, at the end of anaphase, spindle disassembly was observed or final spindle length remained constant for at least 5 minutes

  • c Numbers in parentheses indicate the number of averaged experiments