Table 1.

FRET efficiency

FRET efficiency (%)*
Interphase NE Telophase core region
mCFP-BAF/mVenus-BAF 12.3±8.7 (n=7) 26.7±14.2 (n=15)
mCFP-BAF/mVenus-emerin 15.2±10.6 (n=11) 14.6±13.2 (n=29)
mCFP-BAF/mVenus-emerin m24 ND 2.7±1.35 (n=10)
mCFP-BAF/mVenus-Lamin A 3.1±8.2 (n=10) 23.0±12.0 (n=31)
  • * FRET efficiency (%) = [1–(donor intensity before acceptor photobleaching/donor intensity after acceptor photobleaching)] × 100