Table 2.

Plasmids used in this study

Plasmid Description Reference
pLexN LexA DNA-binding domain (2 μ-TRP1) (Vojtek and Hollenberg, 1995)
pVP16-3 VP16 activation domain (2 μ-LEU2) (Vojtek and Hollenberg, 1995)
pBK111 Mouse vti1b (aa1-128) in pLexN (Chidambaram et al., 2004)
pBK118 Yeast Vti1p (aa1-115) in pLexN (Chidambaram et al., 2004)
pBK130 ENTH-domain of rat epsinR (aa1-162) in pVP16-3 (Chidambaram et al., 2004)
pKW3 ENTH-domain of yeast Ent3p (aa1-172) in pVP16-3 (Chidambaram et al., 2004)
pBK160 ENTH-domain of yeast Ent5p (aa1-172) in pVP16-3 (Chidambaram et al., 2004)
pBK165 Yeast Syn8p (aa1-169) in pLexN This study
pBK171 Yeast Pep12p (aa1-200) in pLexN This study
pBK172 Yeast Tlg1p (aa 1-137) in pLexN This study
pJZ6 Yeast Vam7 (aa 1-255) in pLexN This study
pJZ9 Yeast Pep12p (aa1-200) with F20L in pLexN This study
pJZ10 Yeast Pep12p (aa1-200) with deletion of R19-F26 in pLexN This study
pNK4 Yeast Ykt6p (aa 1-140) in pLexN This study
pBK193 Rat syntaxin 7 (aa1-161) in pLexN This study
pBK179 Rat syntaxin 8 (aa1-142) in pLexN This study
pFvM112 E. coli expression vector pET28-b encoding 6His-Vti1p (aa1-194) (Fischer von Mollard et al., 1997)
pFvM135 E. coli expression vector pET28-b encoding 6His-Pep12p (aa1-268) This study
pFN3 E. coli expression vector pET28-b encoding 6His-Tlg1p (aa1-137) This study
pFN6 E. coli expression vector pET28-b encoding 6His-Syn8p (aa1-169) This study
pKW5 ENTH-domain Ent3p (aa1-172) with C-terminal Strep tag in pASK-IBA3 (Chidambaram et al., 2004)
pRCP59 pGEX 3x encoding GST-Pep12p (aa 1-263) (Chidambaram et al., 2004)
pSN55 A-ALP in CEN vector pRS316 (Nothwehr, 1993)
pSK3 pET28-b encoding 6His-Pep12p (aa1-268) with F20L This study
pSK4 pET28-b encoding 6His-Pep12p (aa1-268) with deletion of R19-F26 This study
pTW1 pET28-b encoding 6His-rat syntaxin 7 (aa1-161) This study
pTW2 pET28-b encoding 6His-rat syntaxin 8 (aa1-142) This study
pJZ11 PEP12 (258 bp 5′ UTR, 240 bp 3′UTR) F20L in pRS314 (CEN6-TRP1) This study
pNM3 ENTH-domain epsinR (aa1-162) with C-terminal Strep tag in pASK-IBA3 (Chidambaram et al., 2004)
pBK38 E. coli expression vector pQE32 encoding 6His-vti1b (aa1-207) (Antonin et al., 2000b)
pBK39 E. coli expression vector pQE32 encoding 6His-vti1a (aa1-187) (Antonin et al., 2000b)