Sequences and amplification conditions of primers used for real-time PCR quantification of DNA with the LightCycler
| Primer name | Sequence (5′-3′) | Amplicon size (bp) | TAnnealing (°C) |
|---|---|---|---|
| LB2-F | GGCTGGCATGGACTTTCATTTCAG | 232 | 66 |
| LB2-R | GTGGAGGGATCTTTCTTAGACATC | ||
| LB2C-F | GTTACCAGTCAGGCGCATGGGCC | 240 | 66 |
| LB2C-R | CCATCAGGGTCACCTCTGGTTCC | ||
| Myc11-F | TATCTACACTAACATCCCACGCTCTG | 192 | 62 |
| Myc11-R | CATCCTTGTCCTGTGAGTATAAATCATCG | ||
| Myc1-F | TCTCAACCTCAGCACTGGTGACA | 248 | 60 |
| Myc1-R | GACTTTGCTGTTTGCTGTCAGGCT |
Names and sequences of primers used for real-time quantification of DNA with the LightCycler (Roche Diagnostics). The primer sets correspond to the amplification regions shown at the maps of the lamin B2 and c-myc origins (see Fig. 3A). `F' and `R' designate the forward and reverse primers, respectively. The size of the PCR products in base pairs (bp) and the annealing temperature (TAnnealing) used in the PCR cycling conditions in °C is also indicated.