Primers used for real-time PCR
| Primer sequence (5′-3′) | ||
|---|---|---|
| Gene | Forward | Reverse |
| Mouse | ||
| Cripto | GCCTATGGGATTCCCTTCC | ACAGCGGGATACAGGGACT |
| Hes1 | ACACCGGACAAACCAAAGAC | CGCCTCTTCTCCATGATAGG |
| Hes5 | GATGCTCAGTCCCAAGGAGA | AGCTTCAGCTGCTCTATGCTG |
| Hesr1 | CATGAAGAGAGCTCACCCAGA | CGCCGAACTCAAGTTTCC |
| Hesr2 | GTGGGGAGCGAGAACAATTA | GTTGTCGGTGAATTGGACCT |
| Hesr3 | CTGAATTGCGACGATTGGT | GCAAGACCTCAGCTTTCTCC |
| Nodal | CCAACCATGCCTACATCCA | CACAGCACGTGGAAGGAAC |
| Sox1 | GTGACATCTGCCCCCATC | GAGGCCAGTCTGGTGTCAG |
| Wnt1 | TACTGGCACTGACCGCTCT | CTTGGAATCCGTCAACAGGT |
| Wnt3 | CTCGCTGGCTACCCAATTT | GAGGCCAGAGATGTGTACTGC |
| β-actin | CTAAGGCCAACCGTGAAAAG | ACCAGAGGCATACAGGGACA |
| Human | ||
| BF1 | TACTACCGCGAGAACAAGCA | TCACGAAGCACTTGTTGAGG |
| CHX10 | GCTGGACACCAGCCAGAC | GCAGATTTGGACATTTTTCGAT |
| CRALBP | AGATCTCAGGAAGATGGTGGAC | GAAGTGGATGGCTTTGAACC |
| CRX | CACCAGGCTGTGCCCTAC | CTTCCAGCTCCTCCAGTTG |
| GBX2 | AAAGAGGGCTCGCTGCTC | ATCGCTCTCCAGCGAGAA |
| HOXB4 | TGGATGCGCAAAGTTCAC | GCTGGACACCAGCCAGAC |
| IRX3 | AAAAGTTACTCAAGACAGCTTTCCA | GAAATTCCTTCTCCAGCTCCA |
| MITF | AGAGTCTGAAGCAAGAGCACTG | TGCGGTCATTTATGTTAAATCTTC |
| NANOG | ATGCCTCACACGGAGACTGT | AGGGCTGTCCTGAATAAGCA |
| OCT3/4 | GCAAAACCCGGAGGAGGAGTC | CCACATCGGCCTGTGTATATC |
| PAX6 | TCACCATGGCAAATAACCTG | CAGCATGCAGGAGTATGAGG |
| RCVRN | TAACGGGACCATCAGCAAG | CCTCGGGAGTGATCATTTTG |
| RPE65 | CAATGGGTTTCTGATTGTGGA | CCAGTTCTCACGTAAATTGGCTA |
| RX | GGCAAGGTCAACCTACCAGA | CTTCATGGAGGACACTTCCAG |
| SIX3 | CCGGAAGAGTTGTCCATGTT | CTCCTCCAGCGTCTCACAG |
| β-actin | ATTGGCAATGAGCGGTTC | GGATGCCACAGGACTCCA |