Table 1.

CFTR is immobile when the ERM-binding capacity of EBP50 is intact and CFTR anchorage is independent of CBP

shRNA-CBP (3T3) Random sequence (3T3) WT-EBP50 (C3H) EGFP–DN-EBP50 (C3H)
Degree of immobility* (%) >99 >99 >99 2.0
  • * The movement of CFTR-bound Qdots was recorded for 1 minute and the mobility was assessed by visual inspection and TA detection software if not completely immobile. In cells other than EGFP-DN-EBP50 transfectants, 99% or more CFTR-bound Qdots were visually immobile throughout the entire duration