Table 1.

Percentage contribution of different tissues to AMP gene expression following infection in wild-type (hmlGAL4) or ablated larvae (hmlGAL4-UAS-GFP; UASice2-2)

Fat body Haemolymph Carcass
Genotype % ± % ± % ±
Drosomycin
   Infected hmlGAL4 69.0 10.0 24.0 5.6 7.0 4.3
   Infected ablated 81.5 6.7 14.6 6.7 3.9 1.0
Diptericin
   Infected hmlGAL4 76.9 6.4 15.9 4.9 7.2 2.7
   Infected ablated 79.2 11.3 10.3 5.8 10.5 5.5
  • Data represent three independent experiments ± s.d. In each case values for whole larvae were set at 100%. See Materials and Methods for experimental details. Diptericin was assayed 6 hours following infection with E. coli while Drosomycin was assayed 12 hours post-infection with Micrococcus luteus. The most significant contribution of AMP for haemocytes (a maximum of 24% for Drosomycin) could not account for the extensive reduction seen in our most haemocyte-depleted lines, where AMP expression in whole larvae was reduced by 85% (see Fig. 2A)