Table 1.

Complete list of positive hits from the genetic dsRNA screen

Screen category/molecular functionCG numberNameNotum adult phenotype
Excess of organs: lateral inhibition defect [Notch signalling loss-of-function (LOF)-like]Sensory organ defect: Notch signalling gain-of-function (GOF)- or loss-of-function (LOF)-like phenotypes
Coat components and accessory proteins
    Clathrin complexCG6948Clathrin light chainLOF: excess of organs (weak)n.o.
    Clathrin bindingCG2520like-AP180LOF: excess of organs (weak)n.o.
    Clathrin adaptor complex (AP-1)CG9113AP-1γn.o.GOF: excess of socket cells, double shafts with socket cell
    Clathrin adaptor complex (AP-1)CG5864AP-1σn.o.GOF: excess of socket cells, double shafts with socket cell
    Clathrin adaptor complex (AP-1)CG9388AP-47n.o.GOF: excess of socket cells, double shafts with socket cell
    Clathrin adaptor complex (AP-2)CG7057AP-50n.o.GOF: excess of socket cells, double shafts with socket cell
    Clathrin adaptor complex (AP-2)CG4260α-adaptinn.o.GOF: excess of socket cells, double shafts with socket cell
    Clathrin adaptor complex (AP-1 and AP-2)CG12532β-adaptinn.o.GOF: excess of socket cells
    Unknown (AP-1 accessory protein: putative orthologue of S. cerevisiae Laa1p)CG2747CG2747n.o.GOF: excess of socket cells, double shafts with socket cell
Lipid microdomain organization/interactors
    Multispan membrane protein with unknown functionCG7740prominin-liken.d.LOF: bristle loss
    TetraspaninCG10742Tetraspanin 3ALOF: excess of organs (weak)n.o.
    TetraspaninCG32136Tetraspanin 68CLOF: excess of organs*LOF: bristle loss
    TetraspaninCG9496Tetraspanin 29 Fbn.d.LOF: bristle loss
    TetraspaninCG12847Tetraspanin 42Ecn.d.LOF: bristle loss
    TetraspaninCG12143Tetraspanin 42Ejn.d.LOF: bristle loss
    TetraspaninCG12841Tetraspanin 42Ekn.d.LOF: bristle loss
    TetraspaninCG9033Tetraspanin 47Fn.d.*LOF: bristle loss
    TetraspaninCG9494Tetraspanin 29 Fan.o.GOF: excess of socket cells
Cytoskeleton, regulators or interactors
    Actin bindingCG5695Jaguarn.d.GOF: excess of socket cells
    Actin bindingCG1520WASpn.d.LOF: bristle loss
    Actin binding; microtubule-associated proteinCG6450lava lampLOF: excess of organsn.o.
    WASp activation complexCG4931specifically Rac1-associated protein 1/CYFIPLOF: excess of organs*LOF: bristle loss
    Arp2/3 actin binding complexCG4560Arpc3ALOF: excess of organsn.o.
    Arp2/3 actin binding complexCG10954Arc-p34LOF: excess of organsLOF: bristle loss, double shafts
    Arp2/3 actin binding complexCG8978Suppressor of profilin 2/Arpc1n.d.LOF: bristle loss
    GTPase activity; actin binding, microtubule-associated proteinCG8705peanutn.d.LOF: bristle loss
    GTPase activity; microtubule-associated proteinCG4173Septin 2n.d.LOF: bristle loss
    GTPase activity; microtubule-associated proteinCG2916Septin 5n.d.*LOF: bristle loss
    DNA processing (replication)CG1584Origin recognition complex subunit 6n.d.LOF: bristle loss, double shafts without socket cell
    RNA processing (Transcription factor)CG7238Septin interacting protein 1n.d.LOF: bristle loss
    Dynein complexCG6998cut upn.d.LOF: bristle loss
    Epsin-likeCG42250liquid facets-Relatedn.d.*LOF: bristle loss
    Microtubule-associated proteinCG3265Eb1n.d.LOF: bristle loss
Small GTPases, GEF/GAP or effectors
    GTPase activity (Rab)CG17515Rab21LOF: excess of organs*LOF: bristle loss
    GTPase activity (Rab)CG9575Rab35LOF: excess of organs (weak)LOF: bristle loss
    GTPase activity (Rab)CG5771Rab-protein 11n.d.LOF: bristle loss
    GTPase activity (Ras)CG8416Rho1n.d.LOF: bristle loss
    GTPase activity (ARF)CG11027ADP ribosylation factor 102Fn.d.*LOF: bristle loss
    GTPase activity (ARF)CG7435ADP ribosylation factor 84Fn.o.*GOF: excess of socket cells
    GTPase (Rap) activator activity (GAP)CG6975gigasLOF: excess of organs (weak)LOF: bristle loss
    GTPase (Rho) activator activity (GAP)CG13345tumbleweedn.d.LOF: bristle loss
    Guanyl-nucleotide exchange factor activity (GEF)CG7787CG7787LOF: excess of organs*LOF: bristle loss, double shafts
    Guanyl-nucleotide exchange factor activity (GEF)CG15797ric8aLOF: excess of organsn.o.
    Guanyl-nucleotide exchange factor activity (GEF)CG9491Gef26LOF: excess of organs (weak)LOF: bristle loss
    Guanyl-nucleotide exchange factor activity (GEF)CG31048CG31048n.d.LOF: bristle loss
    Guanyl-nucleotide exchange factor activity (GEF)CG32239Guanine nucleotide exchange factor GEF64Cn.d.LOF: bristle loss
    Guanyl-nucleotide exchange factor activity (GEF)CG8114Pebblen.d.LOF: bristle loss
    GTPase (Rab5) effector proteinCG8506Rabenosyn-5n.d.LOF: bristle loss
    GTPase (Ras) bindingCG10255Lap1n.d.LOF: bristle loss
Ubiquitylation/deubiquitylation
    Ubiquitin ligase complex (SCF); microtubuleassociated proteinCG16983skpAn.d.LOF: bristle loss, double shafts
    Ubiquitin ligase complex (SCF)CG8881skpBn.d.LOF: bristle loss
    Ubiquitin ligase complex (SCF)CG12700skpDn.d.LOF: bristle loss
    Ubiquitin ligase complex (SCF)CG11942skpEn.d.LOF: bristle loss
    Ubiquitin ligase (E3)CG5841mind bomb 1LOF: excess of organsn.o.
    Ubiquitin ligase (E3)CG11861Cullin-3/Guftagun.d.*LOF: bristle loss, double shafts
    Ubiquitin ligase (E3)CG11988neuralizedn.d.LOF: bristle loss, double shafts
ESCRT components and interactors
    Vacuolar protein sorting (Class E/ESCRT-0)CG2903Hepatocyte growth factor regulated tyrosine kinase substraten.d.LOF: bristle loss, double shafts
    Vacuolar protein sorting (Class E/ESCRT-0)CG6521Signal transducing adaptor moleculeLOF: excess of organs*GOF: excess of socket cells?
    Vacuolar protein sorting (Class E/ESCRT-I)CG12770Vacuolar protein sorting 28n.o.GOF: excess of socket cells
    Vacuolar protein sorting (Class E/ESCRT-II)CG14750Vacuolar protein sorting 25LOF: excess of organsLOF: bristle loss
    Vacuolar protein sorting (Class E/ESCRT-III)CG4071Vacuolar protein sorting 20LOF: excess of organsLOF: bristle loss
    Vacuolar protein sorting (Class E/ESCRT-III)CG4108Chmp1LOF: excess of organsGOF: excess of socket cells
    Vacuolar protein sorting (Class E/ESCRT-III)CG14542Vps2LOF: excess of organs*GOF: excess of socket cells, double shafts
     ATPaseCG6842Vacuolar protein sorting 4n.o.GOF: excess of socket cells?
Membrane recognition and/or fusion
    Exocyst complexCG6095Exo84LOF: excess of organs*LOF: bristle loss and GOF: excess of socket cells, double shafts
    Exocyst complexCG8843Sec5n.d.*GOF: double shafts with socket cell, loss of shaft cells
    Exocyst complexCG5341Sec6n.d.*GOF: loss of shaft cells
    Exocyst complexCG7034Sec15LOF: excess of organs*LOF: bristle loss
    SNAP receptor activity (SNARE)CG1515lethal (1) G0155n.d.*LOF: bristle loss
    SNAP receptor activity (SNARE)CG4109Syntaxin 8n.d.LOF: bristle loss
    SNAP receptor activity (v-SNARE)CG12210synaptobrevinn.d.LOF: bristle loss, double shafts
    SNAP receptor activity (t-SNARE)CG5081Syntaxin 7/AvalancheLOF: excess of organsGOF: excess of socket cells
ATPases
    ATPaseCG9931NEM-sensitive fusion protein 2n.d.*LOF: bristle loss
    Vacuolar ATPase (subunit c)CG3161Vacuolar H+ ATPase subunit 16-1n.d.LOF: bristle loss
    Vacuolar ATPase (subunit c)CG32089Vacuolar H+ ATPase subunit 16-2n.d.LOF: bristle loss
    Vacuolar ATPase (subunit c′)CG7007Vacuolar H+ ATPase subunit PPA1-1LOF: excess of organsLOF: bristle loss
    Vacuolar ATPase (subunit A)CG3762Vacuolar H+ ATPase subunit 68-2n.d.LOF: bristle loss
    Vacuolar ATPase (subunit E)CG1088Vacuolar H+ ATPase 26kD E subunitn.d.LOF: bristle loss
    Vacuolar ATPase (subunit G)CG6213Vacuolar H+ ATPase G-subunitn.d.LOF: bristle loss
Various
    Calcium channel activityCG34146bruchpilotn.d.LOF: bristle loss
    Calcium ion bindingCG9126Stromal interaction moleculen.d.*LOF: bristle loss, double shafts
    Catalytic activity: Notch fucosyltransferaseCG12366O-fucosyltransferase 1n.d.*LOF: bristle loss, double shafts
    Catalytic activity: Notch glucosyltransferaseCG31152rumin.d.LOF: bristle loss
    Catalytic activity: Notch γ-secretaseCG18803presenilinn.d.LOF: bristle loss
    Catalytic activity: histone methyltransferaseCG10955Rtf1n.d.LOF: bristle loss
    Guanylate protein kinaseCG31349polychaetoidLOF: excess of organsn.o.
    Serine/threonine protein kinase; chromosomal passenger complex (CPC)CG6620IplI-aurora-like kinase/Aurora Bn.d.LOF: bristle loss
    Protein binding: TGFβCG14026thickveinsLOF: excess of organsn.o.
    Protein bindingCG9695DisabledLOF: excess of organsn.o.
    Protein binding: Notch ligand activityCG3619DeltaLOF: excess of organsLOF: bristle loss
    Protein binding: Notch receptor activityCG3936Notchn.d.LOF: bristle loss
    Protein binding: NotchCG31020Sanpodon.d.LOF: bristle loss, double shafts
    Protein bindingCG9834endophilin Bn.d.LOF: bristle loss
    Protein bindingCG5820Gp150n.d.LOF: bristle loss
Cell polarity
    Myosin complexCG15792zippern.d.LOF: bristle loss
    Myosin bindingCG2671lethal (2) giant larvaen.o.GOF: excess of socket cells
    Protein bindingCG5055bazookan.d.LOF: bristle loss
    Protein bindingCG6383crumbsn.d.LOF: bristle loss
    Protein bindingCG5884par-6n.o.GOF: excess of socket cells
    Serine/threonine protein kinaseCG3068Aurora/aurora ALOF: excess of organsGOF: double shafts with socket cell
Novel unknown function
    DNA processing (DNA helicase)CG18013Psf2n.d.LOF: bristle loss, double shafts without socket cell
    Microtubule-associated proteinCG8014Receptor mediated endocytosis 8n.d.LOF: bristle loss
    Protein bindingCG6834CG6834n.d.LOF: bristle loss
    Protein binding: NotchCG2863Notchlessn.d.LOF: bristle loss
    RNA processing (snoRNA binding)CG10341CG10341n.o.GOF: double shafts with socket cell, loss of shaft cells
    RNA processing (transcription factor binding)CG7583C-terminal binding proteinn.o.GOF: excess of socket cells
    RNA processing (transcription factor)CG5461bunchedn.d.LOF: bristle loss (weak)
    RNA processing (transcription factor)CG4882CG4882n.d.LOF: bristle loss
    RNA processing (transcription factor)CG4029jumeaun.d.LOF: bristle loss, double shafts
    RNA processing (translation elongation factor); microtubule-associated proteinCG11901Ef1γn.d.LOF: bristle loss
    UnknownCG11295lethal-(2)-denticlelessn.d.LOF: bristle loss
    UnknownCG8435CG8435n.d.*LOF: bristle loss
    UnknownCG8639Cirln.d.LOF: bristle loss
  • Genes are listed in order of the screen category that they were initially selected in. The notum adult phenotypes are sub-divided into two major processes controlled by Notch signalling: lateral inhibition regulating the number of sensory organ precursors specified, and sensory organ lineage, which controls the morphology of the adult organs. The adult phenotypes are indicated as LOF pI (excess of organs resulting from a lateral inhibition defect), LOF (loss of Notch signalling-like phenotypes) and/or GOF (gain of Notch signalling-like phenotypes).

  • n.o., no phenotype observed; n.d., phenotype could not be determined because of bald cuticle (phenotype of bristle loss); *lateral inhibition defect detected on pupal notum by immunostaining.