Table 2.

Complete list of genes that affect Notch, Sanpodo and/or Delta subcellular localisation at the pI daughter cell stage after dsRNA induction

Screen categoryCandidateMolecular functionAdult sensory organ phenotype2-cell stage localisation defects of Notch, Sanpodo and/or Delta
Coat components and accessory proteinsCG2747 (putative orthologue of Saccharomyces cerevisiae Laa1p)AP-1 accessory proteinGOFSubapical accumulation of Spdo. Accumulation of Spdo, Notch and Delta at apical interface.
Lipid microdomain organizationTetraspanin 47FTetraspaninLOFCortex accumulation: Delta at basolateral cortex.
Tetraspanin 68CTetraspaninLOFCortex accumulation: Delta at basolateral cortex.
Cytoskeleton, regulators or interactorsspecifically Rac1-associated protein 1 (Sra-1)/CYFIPWASp activation complexLOFLineage reminiscent to two pIIb-like cells.
Origin recognition complex subunit 6 (Orc-6)septin regulatorLOFLineage reminiscent to two pIIb-like cells.
peanut (pnut)septinLOFLineage reminiscent to two pIIb-like cells.
Septin 5 (Sep5)septinLOFLineage reminiscent to two pIIb-like cells.
Septin 2 (Sep2)septinLOFLineage reminiscent to two pIIb-like cells. Cortex accumulation: Spdo, Notch and Delta at apical cortex of anterior cell.
Small GTPases, GEFs/GAPsRab35RabLOFLineage reminiscent to two pIIb-like cells. Cortex accumulation: Spdo, Notch and Delta at apical cortex of anterior cell.
CG7787RabGEFLOFLateral accumulation of Spdo and Delta. Vesicle excess: large donuts Spdo, Notch and Delta positive.
UbiquitylationCullin-3 (Cul-3)/GuftaguE3 ubiquitin ligaseLOFLateral accumulation of Spdo and Delta. Lineage reminiscent to two pIIb-like cells.
ESCRT components and interactorsSignal transducing adaptor molecule (Stam)ESCRT OGOF ?Accumulation of Spdo, Notch and Delta at apical interface. Vesicle excess: Spdo, Notch and Delta positive. Lineage reminiscent to two pIIa-like cells.
Vacuolar protein sorting 28 (Vps28)ESCRT IGOFSubapical accumulation of Spdo. Accumulation of Spdo, Notch and Delta at apical interface. Weak vesicle excess: Spdo, Notch and Delta positive.
Chmp1ESCRT IIIGOFSubapical accumulation of Spdo. Accumulation of Spdo, Notch and Delta at apical interface. Weak vesicle excess: Spdo, Notch and Delta positive.
Vps2ESCRT IIIGOFAccumulation of Spdo, Notch and Delta at apical interface. Vesicle excess: large donuts Spdo, Notch and Delta positive.
Vacuolar protein sorting 4 (Vps4)ATPaseGOFVesicle excess: large donuts Spdo, Notch and Delta positive.
Membrane recognition and/or fusionSyntaxin 7 (Syx7)/Avalanchet-SNAREGOFLineage reminiscent to two pIIa-like cells.
Exo84Exocyst complexLOF and GOFLineage reminiscent to two pIIa-like cells.
Sec6Exocyst complexGOFLineage reminiscent to two pIIa-like cells.
Sec5Exocyst complexGOFLineage reminiscent to two pIIa-like cells.
Vacuolar ATPaseVacuolar H+ ATPase subunit 16 -1 (Vha16-1)vacuolar ATPase (Vo domain, subunit c)LOFLineage reminiscent to two pIIb-like cells.
Vacuolar H+ ATPase subunit 16-2 (Vha16-2)vacuolar ATPase (Vo domain, subunit c)LOFLineage reminiscent to two pIIb-like cells.
VariousO-fucosyltransferase 1FucosyltransferaseLOFCortex accumulation: Delta at basolateral cortex.
Novel unknown functionCG10341RNA biogenesisGOFAccumulation of Spdo, Notch and Delta at apical interface.
lethal-(2)-denticleless (l(2)dtl)unknown functionLOFVesicle excess in anterior cell (Spdo, Notch and Delta positive).
Recycling pathway, unknown functionReceptor mediated endocytosis 8 (Rme-8)Microtubule associated proteinLOFVesicle excess in anterior cell and toward the anterior in posterior cell (Spdo, Notch and Delta positive).
  • Genes are listed in order of the screen category they belong to.