Table 1.

sql-1 affects anterograde IFT velocities in the middle segment of C. elegans cilia

GenotypeCiliary kinesinsIFT-A particle
KAP-1OSM-3CHE-11DAF-10
VelocitynVelocitynVelocitynVelocityn
Wild type0.70±0.101940.70±0.172120.69±0.211180.69±0.16213
kap-10.70±0.16901.17±0.24a130
osm-30.49±0.10a910.70±0.14150
sql-10.59±0.09a,22100.89±0.19a,11920.84±0.19a,11950.67±0.172212
kap-1; sql-11.19±0.19a2191.10±0.19a211
sql-1; osm-30.52±0.08a2070.49±0.07a210
gpa-30.57±0.14a,*1971.04±0.24a,1,*1520.78±0.161,2,*190
sql-1; gpa-30.59±0.11a,22110.86±0.17a,b,12400.70±0.111,2205
gpa-3QL0.56±0.12a,2,*1480.84±0.22a,1,*1780.73±0.231,2,*222
sql-1; gpa-3QL0.60±0.11a,21900.78±0.16c,12140.68±0.162201
kap-1; gpa-3QL1.19±0.32a231
kap-1; sql-1; gpa-3QL0.94±0.23a,e,f,g2040.86±0.18a,f198
sql-1; osm-3; gpa-3QL0.50±0.07a,c202
GenotypeIFT-B particleDynein (cargo)
OSM-1CHE-13IFT-20XBX-1
VelocitynVelocitynVelocitynVelocityn
Wild type0.70±0.262240.70±0.161320.72±0.112100.72±0.16195
kap-11.03±027a,2213
osm-3
sql-10.82±017a,12270.67±0.121,22070.81±0.17a,12150.82±0.16a,1188
kap-1; sql-11.17±0.25a2051.01±0.20a,2211
sql-1; osm-30.50±0.06a2190.52±0.09a204
gpa-30.80±0.20a,1,2,*1960.77±0.231,2,*202
sql-1; gpa-30.69±0.121,22040.79±0.161220
gpa-3QL0.77±0.271,*2010.73±0.231,2,*201
sql-1; gpa-3QL0.69±0.111,22260.62±0.11a,c,d,2208
kap-1; gpa-3QL
kap-1; sql-1; gpa-3QL0.79±0.18f,2211
sql-1; osm-3; gpa-3QL
  • Average anterograde IFT velocities (in µm/second, mean ± s.d.) of KAP-1::GFP, OSM-3::GFP, CHE-11::GFP, DAF-10::GFP, OSM-1::GFP, CHE-13::GFP, IFT-20::GFP and XBX-1::GFP in wild-type and mutant backgrounds. Statistically significant differences (P<0.001) compared with IFT velocities in wild-type animals are indicated by a, compared with gpa-3(lf) animals by b, compared with sql-1(tm2409) animals by c, compared with gpa-3QL animals by d, compared to kap-1; sql-1(tm2409) animals by e, compared with kap-1; sql-1(tm2409) animals by f, and compared with kap-1; gpa-3QL by g. Statistically significant differences (P<0.001) of IFT velocities of each of the GFP markers compared with the velocities of KAP-1::GFP and OSM-3::GFP in the same mutant background are indicated by 1 and 2, respectively. Statistical analysis was performed using an ANOVA, followed by a Bonferroni post hoc test. n, number of IFT particles measured.

  • * Previously described (Burghoorn et al., 2010).