Cilia
- Intraflagellar transport during assembly of flagella of different length in Trypanosoma brucei isolated from tsetse flies
Summary: Investigation of intraflagellar transport in several trypanosome developmental stages extracted from tsetse flies provides new insights into mechanisms controlling flagellum length.
- Diffusion rather than intraflagellar transport likely provides most of the tubulin required for axonemal assembly in Chlamydomonas
Highlighted Article: Using GFP-tagged β-tubulin, we show that most of the tubulin required for axonemal assembly enters Chlamydomonas cilia by diffusion rather than by intraflagellar transport.
- The structure and symmetry of the radial spoke protein complex in Chlamydomonas flagella
Highlighted Article: A 3D structure of the Chlamydomonas flagella radial spoke was obtained using single particle cryo-EM. Combined with cross-linking mass spectrometry and biophysics data, this allowed localization of components in the radial spoke head and neck regions.
- Epb41l5 interacts with Iqcb1 and regulates ciliary function in zebrafish embryos
Summary: EPB41L5 has a previously uncharacterized role in controlling ciliary function through IQCB1 and CEP290. This EPB41L5 function appears to be independent of its function in epithelial morphogenesis.
- Loss of CPAP in developing mouse brain and its functional implication for human primary microcephaly
Summary: Deletion of microcephaly-associated gene Cpap (or Cenpj), leads to cilia loss, neuroprogenitor mislocalization, junctional integrity disruption, massive heterotopia and severe cerebellar hypoplasia in developing mouse brain.
- Cytoplasmic dynein-2 at a glance
Summary: We discuss current understanding of the cytoplasmic dynein-2 microtubule motor, and its roles in cilia assembly and function.
- LAX28 is required for the stable assembly of the inner dynein arm f complex, and the tether and tether head complex in Leishmania flagella
Summary: The inner dynein arm f (or l1) complex is required for coordinating flagellar motility. We show that LAX28 is needed for its function and localization in the flagellum of Leishmania mexicana.
- Centriolar satellite biogenesis and function in vertebrate cells
Summary: A review of centriolar satellite biogenesis and function discussing satellite composition and regulation, along with their involvement in cilium assembly and function, proteostasis and other cellular processes.
- MICAL-L1 coordinates ciliogenesis by recruiting EHD1 to the primary cilium
Highlighted Article: A pool of centriolar γ-tubulin and/or α/β-tubulins anchor MICAL-L1 to the centriole, where it might recruit EHD1 to promote ciliogenesis.
- Ahi1 promotes Arl13b ciliary recruitment, regulates Arl13b stability and is required for normal cell migration
Summary: The Joubert syndrome-causing protein, Ahi1, acts as a gatekeeper in regulating primary cilia protein composition and length. Ahi1 also regulates Arl13b stability, Shh signaling and cell migration.