Cilia
- The Chlamydomonas flagellar membrane glycoprotein FMG-1B is necessary for expression of force at the flagellar surface
Summary: The Chlamydomonas flagellar membrane glycoprotein FMG-1B is responsible for coupling the motor machinery of IFT to an extracellular substrate to generate flagellar surface motility (whole-cell gliding and microsphere movement).
- High-speed multifocal plane fluorescence microscopy for three-dimensional visualisation of beating flagella
Summary: A new 3D fluorescence microscopy-based method allows analysis of the high speed beating of cilia and flagella, with potential to answer open questions about Leishmania and Trypanosoma 3D motility.
- Microtubule glycylation promotes attachment of basal bodies to the cell cortex
Highlighted Article: Motile cilia are positioned at the cell cortex by basal bodies (BB). Tubulin glycylation promotes elongation of BB-associated microtubules and structural attachment of BBs to the cortical cytoskeleton.
- Polyubiquitylation of α-tubulin at K304 is required for flagellar disassembly in Chlamydomonas
Summary: In Chlamydomonas, ciliary α-tubulin is labeled with K63 polyubiquitin chains at K304 during ciliary disassembly and transported to cell body by retrograde intraflagellar transport.
- IFT25 is required for the construction of the trypanosome flagellum
Summary: The IFT25 protein of Trypanosoma brucei is required for construction of its flagellum, a situation equivalent to the mouse sperm flagellum but not to primary cilia.
- Rab34 small GTPase is required for Hedgehog signaling and an early step of ciliary vesicle formation in mouse
Summary: Rab34 is required for an early step of ciliary vesicle formation and Hedgehog signaling in vivo.
- RPGRIP1L helps to establish the ciliary gate for entry of proteins
Summary: RPGRIP1L, a key protein of the transition zone that is needed for NPHP4 localization, helps to exclude cytoplasmic proteins from the cilia in Chlamydomonas reinhardtii.
- LRRC45 contributes to early steps of axoneme extension
Summary: LRRC45 at distal appendages is required for early steps of axoneme extension by promoting transition zone establishment, Rab8a recruitment and satellite organisation.
- Phosphatidylinositol 4,5-bisphosphate regulates cilium transition zone maturation in Drosophila melanogaster
Summary: The membrane phospholipid PIP2, and the kinase that produces PIP2, called Skittles, are needed for normal ciliary transition zone morphology and function in the Drosophila male germline.