Cilia
- Cytoplasmic dynein-2 at a glance
Summary: We discuss current understanding of the cytoplasmic dynein-2 microtubule motor, and its roles in cilia assembly and function.
- LAX28 is required for the stable assembly of the inner dynein arm f complex, and the tether and tether head complex in Leishmania flagella
Summary: The inner dynein arm f (or l1) complex is required for coordinating flagellar motility. We show that LAX28 is needed for its function and localization in the flagellum of Leishmania mexicana.
- Centriolar satellite biogenesis and function in vertebrate cells
Summary: A review of centriolar satellite biogenesis and function discussing satellite composition and regulation, along with their involvement in cilium assembly and function, proteostasis and other cellular processes.
- MICAL-L1 coordinates ciliogenesis by recruiting EHD1 to the primary cilium
Highlighted Article: A pool of centriolar γ-tubulin and/or α/β-tubulins anchor MICAL-L1 to the centriole, where it might recruit EHD1 to promote ciliogenesis.
- Ahi1 promotes Arl13b ciliary recruitment, regulates Arl13b stability and is required for normal cell migration
Summary: The Joubert syndrome-causing protein, Ahi1, acts as a gatekeeper in regulating primary cilia protein composition and length. Ahi1 also regulates Arl13b stability, Shh signaling and cell migration.
- The Chlamydomonas flagellar membrane glycoprotein FMG-1B is necessary for expression of force at the flagellar surface
Highlighted Article: The Chlamydomonas flagellar membrane glycoprotein FMG-1B is responsible for coupling the motor machinery of IFT to an extracellular substrate to generate flagellar surface motility (whole-cell gliding and microsphere movement).
- High-speed multifocal plane fluorescence microscopy for three-dimensional visualisation of beating flagella
Summary: A new 3D fluorescence microscopy-based method allows analysis of the high speed beating of cilia and flagella, with potential to answer open questions about Leishmania and Trypanosoma 3D motility.
- Microtubule glycylation promotes attachment of basal bodies to the cell cortex
Highlighted Article: Motile cilia are positioned at the cell cortex by basal bodies (BB). Tubulin glycylation promotes elongation of BB-associated microtubules and structural attachment of BBs to the cortical cytoskeleton.