confocal microscopy
- Experimental toolbox for quantitative evaluation of clathrin-mediated endocytosis in the plant model Arabidopsis
Summary: We present detailed novel quantitative imaging protocols, and review pharmacological and genetic manipulation for the investigation of clathrin-mediated endocytosis at multiple scales in Arabidopsis plants.
- ADAP is an upstream regulator that precedes SLP-76 at sites of TCR engagement and stabilizes signaling microclusters
Highlighted Article: During T cell activation, a cytoskeletal pool of ADAP enters nascent contacts in advance of other adaptors and enhances contact stability and TCR signaling by participating in multivalent signaling complexes.
- Novel cell-penetrating peptide-adaptors effect intracellular delivery and endosomal escape of protein cargos
Highlighted Article: Novel cell-penetrating peptide-calmodulin adaptor proteins effect efficient delivery of user-defined cargo proteins to the cytoplasm of living mammalian cells.
- Two-color fluorescent analysis of connexin 36 turnover: relationship to functional plasticity
Summary: Fluorescent labeling of Cx36-HaloTag revealed that turnover occurs on a different time scale than phosphorylation-driven plasticity. Cx36-HaloTag is also partially mobile within gap junctions.
- Analysis of ER–mitochondria contacts using correlative fluorescence microscopy and soft X-ray tomography of mammalian cells
Highlighted Article: Confocal live-cell imaging combined with correlative cryogenic fluorescence microscopy and soft X-ray tomography provide new insights into the complex process of mitochondrial fission in mammalian cells.