Membrane
- The ubiquitin hydrolase Doa4 directly binds Snf7 to inhibit recruitment of ESCRT-III remodeling factors in S. cerevisiae
Highlighted Article: ESCRT-III remodeling is needed for its membrane scission activity. The Doa4 ubiquitin hydrolase in yeast is shown to interfere during association of ESCRT-III with its remodeling factors.
- Reconstruction of destruction – in vitro reconstitution methods in autophagy research
Summary: Autophagy is a versatile recycling system that ensures turnover of cytoplasmic material. This Review highlights in vitro reconstitution studies that helped to discover fundamental mechanisms of the pathway.
- Mechanical forces on cellular organelles
Summary: Organelles are subjected to mechanical forces within the crowded cytoplasm of eukaryotic cells. We discuss the effect of forces on two organelles, the nucleus and the mitochondrion.
- Lipid-dependence of target membrane stability during influenza viral fusion
Summary: Target membrane integrity during influenza hemagglutinin-mediated lipid mixing between viral and target membrane is dependent upon target membrane spontaneous curvature.