Nuclear lamina
- Investigating cell cycle-dependent gene expression in the context of nuclear architecture at single-allele resolution
Summary: A robust, high-throughput microscopy-based method to investigate nuclear architecture-dependent gene expression at single-cell and single-allele resolution in the context of the cell cycle.
- The PRR14 heterochromatin tether encodes modular domains that mediate and regulate nuclear lamina targeting
Summary: We have identified an evolutionarily conserved nuclear lamina binding domain in the PRR14 nuclear lamina–heterochromatin tethering protein.
- Emerging roles of mechanical forces in chromatin regulation
Summary: This Review discusses the mechanisms by which the nucleus, and chromatin in particular, respond to changes in the mechanical microenvironment of the cell, resulting in architectural and transcriptional alterations, and thereby facilitating cellular adaption.
- Substrate stiffness-dependent regulation of the SRF−Mkl1 co-activator complex requires the inner nuclear membrane protein Emerin
Summary: The nuclear lamina regulates SRF−Mkl1 activity specifically in cells grown within stiff mechanical environments.